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A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System

A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System

DOI:
10.3791/59201-v

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05:28 min

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August 15, 2019

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, , , ,
1Department of Bioengineering and Institute of Nanoscience and Technology,Hanyang University, 2Department of Internal Medicine, Section of Infectious Diseases,Yale University School of Medicine

Chapters

  • 00:04Title
  • 00:28Mouse Positioning
  • 01:23Intranasal Delivery
  • 03:28Results: Distribution of Fluorescently Labeled Peptide and siRNA after Intranasal Administration
  • 04:52Conclusion

Summary

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Here, we present a protocol using a mouse positioning device that enables the appropriate placement of mice for the intranasal administration of a brain-targeting peptide-siRNA formulation enabling effective gene silencing in the central nervous system.

Tags

Keywords: Positioning DeviceIntranasal DeliveryCentral Nervous SystemSiRNAMiceAnesthesiaIntranasal InoculationRVG9R SiRNA ComplexMicropipetteReproducibility

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