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Transient Expression in Red Beet of a Biopharmaceutical Candidate Vaccine for Type-1 Diabetes
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Bioengineering
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JoVE Journal Bioengineering
Transient Expression in Red Beet of a Biopharmaceutical Candidate Vaccine for Type-1 Diabetes

Transient Expression in Red Beet of a Biopharmaceutical Candidate Vaccine for Type-1 Diabetes

DOI:
10.3791/59298-v

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10:16 min

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March 19, 2019

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, , , , , ,
1Department of Biotechnology,University of Verona

Chapters

  • 00:04Title
  • 00:58Red Beet and Spinach Cultivation
  • 01:23Syringe Agroinfiltration
  • 02:58Vacuum Agroinfiltration
  • 04:48Plant Material Processing
  • 05:27Gastric Digestion Simulation and Cell Integrity Analysis
  • 07:22Bioburden Assay
  • 08:00Results: Analysis of the Transient Expression in Red Beet of a Biopharmaceutical Candidate Vaccine for Type-1 Diabetes
  • 09:46Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to produce an oral vaccine candidate against Type 1 diabetes in an edible plant.

Tags

Keywords: Transient ExpressionRed BeetBiopharmaceuticalType-1 DiabetesVaccineVERS ExpressionOral VaccineLyophilized Red Beet LeavesPhoto AntigensOral ToleranceEdible SpeciesRecombinant ProteinAgroinfiltrationPlant Expression VectorsAgrobacterium TumefaciensInfiltration BufferLeaf Infiltration

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