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JoVE Journal
Bioengineering

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Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores
 

Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores

Article DOI: 10.3791/59388-v
April 15th, 2019

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Summary April 15th, 2019

Germinant receptor proteins cluster in ‘germinosomes’ in the inner membrane of Bacillus subtilis spores. We describe a protocol using super resolution microscopy and fluorescent reporter proteins to visualize germinosomes. The protocol also identifies spore inner membrane domains that are preferentially stained with the membrane dye FM4-64.

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