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激发-扫描高光谱成像显微镜,以有效区分荧光信号
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JoVE Journal
Engineering
Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
Please note that all translations are automatically generated.
Click here for the English version.
激发-扫描高光谱成像显微镜,以有效区分荧光信号
DOI:
10.3791/59448-v
•
00:07 min
•
August 22, 2019
•
Joshua Deal
2,3
,
Andrea Britain
3
,
Thomas Rich
3
,
Silas Leavesley
2,3
1
Department of Chemical and Biomolecular Engineering
,
University of South Alabama
,
2
Center for Lung Biology
,
University of South Alabama
,
3
Department of Pharmacology
,
University of South Alabama
Chapters
00:04
Title
00:44
Excitation-Scanning Hyperspectral Imaging Microscopy
04:12
Image Analysis
05:41
Results: Excitation-Scanning Hyperspectral Imaging
06:43
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
光谱成像已成为在单个样品中识别和分离多个荧光信号的可靠解决方案,并可轻松区分感兴趣的信号和背景或自荧光。激励扫描高光谱成像通过减少必要的图像采集时间,同时提高信噪比,改进了该技术。
Tags
Excitation-scanning
Hyperspectral Imaging
Fluorescence Signals
Spectral Imaging Microscopy
Supercontinuum Laser
Spinning Disk
Laser Scanning Confocal Microscope
Fluorescent Labels
Sample Preparation
Image Acquisition
Image Analysis
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