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Modelo de xenoinjerto heterogéneo derivado del paciente del cáncer de páncreas utilizando larvas de pez cebra como anfitriones para la evaluación comparativa de fármacos
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1Department of Biochemistry and Molecular Biology, Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, School of Basic Medical Sciences,Fudan University, 2National Human Genetic Resources Sharing Service Platform (2005DKA21300), 3Fudan University Shanghai Cancer Center, 4Department of Pancreatic Surgery,Fudan University Shanghai Cancer Center, 5Department of Oncology, Shanghai Medical College,Fudan University, 6Pancreatic Cancer Institute,Fudan University
Chapters
- 00:04Title
- 01:28Preparing the Equipment for the Microinjection
- 02:21Preparing Embryos for Transplantation
- 02:56Isolation and Culture of Primary Human Cells from Fresh Surgical Pancreatic Cancer Specimen or Frozen Tissue
- 04:56Labeling the Cells with Lentivirus Expressing Anti-apoptosis Gene BCL2L1 and Different Fluorescent Proteins Separately
- 06:29Injecting Mixed Cell Suspension into the Zebrafish
- 07:10Culture of the Xenografted Zebrafish (zPDX Model), Drug Administrationon the Xenografted Zebrafish, and the Assessment of Tumor Cells/Fibroblasts Viabilities
- 08:11Results: Analysis of the Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae
- 09:12Conclusion
Este protocolo describe los procedimientos de optimización en un modelo de xenoinjerto tumoral doble etiquetado con fluorescencia doble basado en virus utilizando peces cebra larvales como huéspedes. Este modelo heterogéneo de xenoinjerto imita la composición tisular del microambiente del cáncer de páncreas in vivo y sirve como una herramienta más precisa para evaluar las respuestas de los medicamentos en modelos personalizados de xenoinjerto derivado del paciente de pez cebra.
