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Immunology and Infection

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Um modelo de cultura em lote in vitro para estimar os efeitos dos regimes intervencionistas na microbiota fecal humana
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Um modelo de cultura em lote in vitro para estimar os efeitos dos regimes intervencionistas na microbiota fecal humana

Article DOI: 10.3791/59524-v • 07:15 min • July 31st, 2019
July 31st, 2019 •
*1,2,3, *1,2, *1,2, 1,2, 3,4, 5, 1,2
1Department of Internal Medicine- Molecular Medicine, Wake Forest School of Medicine, 2Department of Microbiology and Immunology, Wake Forest School of Medicine, 3Department of Food Science and Technology, Isfahan University of Technology, 4Research Institute for Biotechnology and Bioengineering, College of Agriculture, Isfahan University of Technology, 5Department of Geriatrics and Gerontology, Wake Forest School of Medicine
* These authors contributed equally

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Este protocolo descreve um sistema de fermentação em lote-cultura in vitro de microbiota fecal humana, utilizando inulina (um prebiótico bem conhecido e um dos moduladores de microbiota mais amplamente estudados) para demonstrar o uso deste sistema na estimativa de efeitos de intervenções sobre a composição da microbiota fecal e atividades metabólicas.

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Imunologia e infecção edição 149 anaeróbia cultura de lotes cólon fermentação in vitro microbiota microflora ácidos graxos de cadeia curta pH
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