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JoVE Journal
Immunology and Infection

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Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles
 

Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles

Article doi: 10.3791/59555
April 30th, 2019

Summary April 30th, 2019

We demonstrate single-cell culture of bacteria inside giant vesicles (GVs). GVs containing bacterial cells were prepared by the droplet transfer method and were immobilized on a supported membrane on a glass substrate for direct observation of bacterial growth. This approach may also be adaptable to other cells.

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