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JoVE Journal
Biology

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Fabrication of Amyloid-β-Secreting Alginate Microbeads for Use in Modelling Alzheimer's Disease
 

Fabrication of Amyloid-β-Secreting Alginate Microbeads for Use in Modelling Alzheimer's Disease

Article DOI: 10.3791/59597-v 06:52 min July 6th, 2019
July 6th, 2019

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Summary

This protocol illustrates a cell encapsulation method by rapid physical gelation of alginate to immobilize cells. Obtained microbeads allow controlled and sustained secretion of amyloid-β over time and can be used to study the effects of secreted amyloid-β in in vitro and in vivo models.

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Fabrication Amyloid Alzheimer's Disease Microbeads Protein Release Rate Control Biomaterial Encapsulation Chronic Release In Vitro In Vivo Mechanisms Of Disease New Treatments Controlled System Biomolecules Cell Types Trypsin-EDTA Solution DMEM/F12 Medium Centrifuge HEPES Buffered Saline Cell Concentration
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