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Fabrication of Amyloid-β-Secreting Alginate Microbeads for Use in Modelling Alzheimer's Disease

Article DOI: 10.3791/59597-v • 06:52 min • July 6th, 2019
July 6th, 2019 •
1, 2, 1, 1
1Division of Pharmacy and Optometry, School of Health Studies, Faculty of Biology, Medicine and Health, University of Manchester, 2Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester

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Summary
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This protocol illustrates a cell encapsulation method by rapid physical gelation of alginate to immobilize cells. Obtained microbeads allow controlled and sustained secretion of amyloid-β over time and can be used to study the effects of secreted amyloid-β in in vitro and in vivo models.

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Fabrication Amyloid Alzheimer's Disease Microbeads Protein Release Rate Control Biomaterial Encapsulation Chronic Release In Vitro In Vivo Mechanisms Of Disease New Treatments Controlled System Biomolecules Cell Types Trypsin-EDTA Solution DMEM/F12 Medium Centrifuge HEPES Buffered Saline Cell Concentration
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