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Uso de CRISPR/Cas9 para noquear GM-CSF en células CAR-T
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells
DOI:

07:56 min

July 22, 2019

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Chapters

  • 00:04Title
  • 00:41T Cell Stimulation and Ex Vivo Culture
  • 02:06T Cell Transfection and Transduction
  • 03:35CART19 Cell Expansion
  • 04:48GM-CSFk/o CART19 Production
  • 06:18Results: Successful Knockout of GM-CSF in CAR-T Cells
  • 07:16Conclusion

Summary

Automatic Translation

Aquí, presentamos un protocolo para editar genéticamente las células CAR-T a través de un sistema CRISPR/Cas9.

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