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Immunology and Infection

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Renal Subcapsular Transplantation of 2′-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice

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Immunology and Infection

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JoVE Journal Immunology and Infection

Renal Subcapsular Transplantation of 2′-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice

Renal Subcapsular Transplantation of 2′-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice

DOI:

10.3791/59657-v

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07:39 min

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July 19, 2019

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Jianxue Wang^3,4, Guolin Chen^3,4, Qianwen Cui^3,4, Erfei Song^3,4, Weihua Tao, Wanqun Chen, Cunchuan Wang^3,4, Shiqi Jia^3,4

¹The First Affiliated Hospital,Jinan University, ²Department of Biochemistry and Molecular Biology, School of Basic Medical Science,Jinan University, ³The Institute of Clinical Medicine,Jinan University, ⁴Joint institute of Metabolic Medicine Between Jinan University and University of Hong Kong

Chapters

00:04Title
01:06Embryonic Thymus Isolation
02:54Isolated Embryonic Thymus Culture and Renal Subcapsular Space Establishment
04:36Embryonic Murine Thymus Transplant
05:59Results: Thymus-Transplant Nude Mouse Peripheral Blood T Cell Identification and Evaluation
06:59Conclusion

Summary

Automatic Translation

We provide a simple and efficient method to transplant 2’-deoxyguanosine treated E18.5 thymus into the renal capsule of a nude mouse. This method should aide in the study of both thymic epithelial cells function and T cells maturation.

Tags

Keywords: Renal Subcapsular Transplantation Murine Embryonic Thymus Nude Mice Thymus Transplantation Thymus Epithelial Cell Function T-cell Maturation 18.5 Thymus Transplantation 2′-deoxyguanosine Treatment Thymic Transplantation

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