Kidney Capsule Procedure
Kidney Capsule Procedure - Related Videos
03:00
Extracting Intact Kidney from Mouse: A Technique to Obtain Kidney Without Renal Capsule from Murine Models
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2023
In this video, we describe the procedure for kidney extraction from the retroperitoneal space of the mouse. The extracted kidney can further be microdissected to obtain an intact kidney without a renal capsule and the surrounding fat layer.
10:43
Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice
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Cited by 98 •
2007
Our protocol was developed to cleanly and easily deliver islets or cells under the kidney capsule of mice. Cells are concentrated into pellets in the final tubing used for transplanting the cells under the kidney capsule. The ease of this technique reduces stress to the cells and the mouse.
08:19
The Mouse Isolated Perfused Kidney Technique
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Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
03:30
Preparation of Decellularized Rat Kidney: A Procedure to Generate Acellular Vascularized Whole-organ Scaffold from a Harvested Rat Kidney
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2023
This video describes the preparation of a decellularized rat kidney. The obtained acellular vascular scaffold can serve as a promising platform for tissue engineering of organ grafts.
01:26
Kidney Transplant II: Surgical Procedure
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2025
Preoperative ManagementThe primary goals of preoperative management in kidney transplantation are to optimize the patient’s metabolic state and prepare them for surgery through diet adjustments, necessary dialysis, and tailored medical treatment. This phase also involves comprehensive infection screening and patient education about the surgical procedure and postoperative care to improve outcomes and adherence.Medical ManagementA comprehensive evaluation is required for both the living donor...
FAQs
Retention beneath the fibrous capsule keeps the implanted material at a defined anatomical site, while the surrounding kidney tissue provides local vascular access. This combination lets investigators examine the material in a living host and follow graft survival together with nearby inflammation, immune-cell infiltration, or tissue development.
Closure around the implant helps maintain its placement at the selected site, while the capsule’s relationship with underlying kidney tissue creates a local setting in which host responses can be observed. Studies can therefore connect graft retention with vascular access, immune-cell infiltration, inflammation, tissue development, and pathogen responses.
Interpretation depends on both what is placed beneath the capsule and how the host responds to it. Cells, tissues, organoids, and other biological materials can be examined for survival or development, while immune-cell infiltration and inflammation reveal host reactions. In infection studies, pathogen responses provide an additional outcome for evaluating implant-host interactions.
Researchers expose the kidney, gently separate the fibrous capsule from the underlying tissue, insert the graft or other biological material, and allow the capsule to close around it. This sequence establishes the intended subcapsular location and supports subsequent observation of graft survival, local immune activity, inflammation, pathogen responses, or tissue development.
The approach can accommodate cells, tissues, organoids, other biological materials, and biomaterials placed beneath the kidney capsule. This range allows investigators to use the same defined in vivo site for questions involving tissue development, transplantation, therapeutic strategies, or host responses to implanted material.
Researchers can assess whether a graft survives, whether immune cells enter the implant region, and whether local inflammation develops. The site also supports evaluation of tissue development and pathogen responses. Together, these observations provide information about how implanted material behaves and how the living host responds over the course of a study.
Its value comes from combining a defined implantation site with access to a living host. Investigators can examine immune-cell infiltration and inflammation around grafts, assess pathogen responses, and relate those findings to graft survival or tissue development. The same framework also supports transplantation, biomaterial, and therapeutic-strategy research.