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Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
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JoVE Journal Neuroscience
Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

DOI:
10.3791/59797-v

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07:35 min

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August 06, 2019

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,
1Laboratory of RNA Biology,Institute of Molecular Genetics of the Czech Academy of Sciences, 2Department of Genetics and Microbiology, Faculty of Science,Charles University

Chapters

  • 00:04Title
  • 00:34Injection
  • 02:54Cell Fixation and Staining
  • 04:07Microscopy
  • 05:09Results: Analysis of Spliceosomal snRNA Localization in Human Hela Cells
  • 07:00Conclusion

Summary

Automatic Translation

Automatic Translation

Biogenesis of spliceosomal snRNAs is a complex process involving various cellular compartments. Here, we employed microinjection of fluorescently labelled snRNAs in order to monitor their transport inside the cell.

Tags

Keywords: Spliceosomal SnRNAMicroinjectionRNA LocalizationCajal BodiesHeLa CellsFluorescently Labeled RNARNA SequenceNuclear StructuresAdherent CellsMicroloaderMicroinjectorMicroscopeObjectiveCell ContactLower LimitCell Injection

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