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高产细胞外囊泡制剂的纯化远离病毒

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

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高产细胞外囊泡制剂的纯化远离病毒

DOI:

10.3791/59876-v

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00:07 min

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September 12, 2019

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Catherine DeMarino*¹, Robert A. Barclay*¹, Michelle L. Pleet, Daniel O. Pinto, Heather Branscome², Siddhartha Paul, Benjamin Lepene, Nazira El-Hage, Fatah Kashanchi

¹Laboratory of Molecular Virology, School of Systems Biology,George Mason University, ²American Type Culture Collection (ATCC), ³ATCC Cell Systems, ⁴Ceres Nanosciences, Inc, ⁵Department of Immunology and Nano-medicine, Herbert Wertheim College of Medicine,Florida International University

Chapters

00:04Title
01:00Filtration and Precipitation of Extracellular Vesicles
02:25Construction of a Density Gradient and Enrichment of EV Fractions
04:06Preparation of Nanoparticle Pellet for Downstream Assay
05:05Results: Increased EV Yield and Characterization of EVs
06:09Conclusion

Summary

Automatic Translation

该协议通过结合EV沉淀、密度梯度超离和颗粒捕获，将细胞外囊泡（EV）与高效和屈服的病毒分离，从而简化工作流程和减少启动体积要求，导致可重复的制剂用于所有EV研究。

Tags

Extracellular Vesicles EVs Virus-free EV Preparations EV Purification Ultracentrifugation PEG Precipitation Iodixanol Density Gradient Nanoparticle Enrichment

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