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Developmental Biology
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从多能干细胞中无血清自由生产三维人类肝球
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
从多能干细胞中无血清自由生产三维人类肝球
DOI:
10.3791/59965-v
•
06:57 min
•
July 20, 2019
•
Balta Lucendo-Villarin
,
Hassan Rashidi
2
,
Sharmin Alhaque
3
,
Lena Fischer
4
,
Jose Meseguer-Ripolles
,
Yu Wang
,
Cliona O’Farrelly
,
Michael Themis
,
David C. Hay
1
MRC Centre for Regenerative Medicine
,
University of Edinburgh
,
2
UCL Great Ormond Street Institute of Child Health
,
University College London
,
3
Division of Biosciences, Department of Life Sciences, College of Health and Life Sciences
,
Brunel University London
,
4
School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute
,
Trinity College Dublin
Chapters
00:04
Title
00:31
Preparation of Agarose Microplate Molds
01:12
Seeding Human Pluripotent Stem Cells into Agarose Microwell Plates
03:07
Differentiating hPSCs to 3D Hepatospheres
05:28
Results: Hepatosphere Structure and Function
06:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种使用定义的培养系统和细胞自组装从人类多能干细胞中产生肝球的方法。该协议可重复在多个细胞系中,具有成本效益,并允许生产稳定的人类肝球用于生物医学应用。
Tags
3D Hepatospheres
Pluripotent Stem Cells
Serum-free
Agarose Molds
Cell Suspension
Rock Inhibitor
Cell Culture
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