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Journal / Biochemistry / En semi-High-gennemløb tilpasning af NADH-koblede …
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Biochemistry

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JoVE Journal Biochemistry
En semi-High-gennemløb tilpasning af NADH-koblede ATPase assay til screening små molekyle hæmmere
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En semi-High-gennemløb tilpasning af NADH-koblede ATPase assay til screening små molekyle hæmmere

Article DOI: 10.3791/60017-v • 10:28 min • August 17th, 2019
August 17th, 2019 •
1,2, 1,2, 3, 2, 1,2
1Department of Molecular Medicine, The Scripps Research Institute, 2Department of Neuroscience, The Scripps Research Institute, 3Laboratory of Molecular Physiology, NHLBI, National Institutes of Health

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En nicotinamid-adenin dinucleotid (NADH)-koblet ATPase-analyse er blevet tilpasset til halvhøj gennemløbs screening af små molekyle myosin-hæmmere. Denne kinetiske analyse køres i et 384-brønd mikroplade format med samlet reaktions volumen på kun 20 μL pr. brønd. Platformen bør gælde for stort set alle ADP-producerende enzymer.

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Biokemi ATPase assay NADH fluorescens semi High-gennemløb screening inhiberende konstant myosin
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