Name: Murine Model of Controlled Cortical Impact for the Induction of Traumatic Brain Injury (Video) | JoVE Journal
Uploaded: 2019-08-16T14:45:00.000+00:00
Duration: 5 min 1 s
Description: Learn controlled cortical impact for inducing traumatic brain injury in mice using this standardized protocol. This reproducible method enables behavioral, imaging, and molecular analyses of TBI mechanisms and recovery.

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5 minutes

This work was supported by National Institutes of Health Grant GM117341 and The American College of Surgeons C. James Carrico Research Fellowship to S.J.S.

All procedures were approved by the Northwestern University Institutional Animal Care and Use Committee. C57BL/6 mice were purchased from the Jackson Laboratory and group housed at a barrier facility at the Center for Comparative Medicine at Northwestern University (Chicago, IL). All animals were housed in 12/12 h light/dark cycle with free access to food and water.
1. Induce anesthesia
<ol>
	<li>Anesthetize the mouse with ketamine (125 mg/kg) and xylazine (10 mg/kg) injected intraperitoneally.</li>
</ol>
2. Vital signs monitoring every 15 min
<ol>
	<li>Monitor temperature, respiratory rate, and skin color. The mouse should feel warm to the touch. The skin should appear pink and well perfused. Respiratory rate should range 50&#8211;70 breaths per minute.</li>
</ol>
3. Pre-surgical procedures
<ol>
	<li>Weigh all the mice on the day prior to injury induction.</li>
	<li>Sterilize one set of surgical instruments by autoclaving for each experimental subject. Sterilize the impacting device before use.&#160;</li>
	<li>Prepare a recovery cage by placing a clean cage over an electric heating pad set to &#8220;low&#8221; setting and positioned in a manner such that the mice can move away from the heat once ambulatory.</li>
	<li>Set up the operating theater within a sterilized laminar flow hood.
	<ol>
		<li>Position the stereotaxic operating frame.</li>
		<li>Attach the impacting device to the stereotaxic frame.</li>
		<li>Set the actuating device with the desired biomechanical parameters for velocity and dwell time. 
		NOTE: In this protocol a severe brain injury is described utilizing a 3 mm diameter impact tip via a 5 mm diameter craniectomy with the velocity set at 2.5 m/s and a dwell time of 0.1 s. A wide range of biomechanical parameters may be used to induce the full spectrum of TBI.</li>
	</ol>
	</li>
	<li>Don new personal protective equipment and sterile gloves.</li>
	<li>Shave the fur from the operative site using electric clippers.</li>
	<li>Apply protective opthalmic ointment to the eyes of the mouse to prevent corneal injury and drying.</li>
	<li>Place the mouse into the operating theater.</li>
	<li>Prep the skin with an iodine based surgical scrub alternated with alcohol three times.</li>
</ol>
4. Application of controlled cortical impact
<ol>
	<li>Incise the scalp 1 cm in the midline with a scalpel exposing the skull.</li>
	<li>Position the mouse within a stereotaxic operating frame by securing the bilateral temporal bones between miniature ear bars and locking the incisors within an incisor clamp creating a stable three-point-hold on the mouse head.</li>
	<li>Retract the scalp away from the operative site with a hemostat or locking forceps to ensure the scalp does not come in contact with the drill bit during craniectomy.</li>
	<li>Identify the sagittal and coronal sutures on the exposed skull. 
	NOTE: This protocol centers the craniectomy 2 mm left of the sagittal suture and 2 mm rostral to the coronal suture.</li>
	<li>Perform a craniectomy using a drill with a trephine drill bit.
	<ol>
		<li>To perform the craniectomy, first activate the drill at maximum speed and then apply the trephine drill bit perpendicular to the skull at the site of craniectomy.</li>
		<li>Apply gentle, even pressure to the drill once contact is made with the skull. A slight &#8220;give&#8221; will be felt once the drill penetrates through the skull. Do not penetrate the underlying dura. 
		NOTE: This protocol utilizes a 5 mm trephine drill bit to perform the craniectomy.</li>
	</ol>
	</li>
	<li>Use forceps and a small gauge hypodermic needle to remove the bone flap, fully exposing the underlying dura mater.</li>
	<li>Rotate the impactor tip into the operative field and lower it until it makes contact with the exposed dura mater. Once contact is made the instrument&#8217;s contact sensor will make an audible tone to alert the surgeon that contact has been made. This will mark the zero point from which the deformation depth is set. 
	NOTE: This protocol utilizes a 3 mm impacting tip to generate a severe injury. Tips as small as 1 mm may be used to apply more localized injury.</li>
	<li>Retract the impacting tip and set the desired impact depth by lowering the impactor position on the stereotaxic frame. 
	NOTE: In this protocol we describe a severe injury by setting the deformation depth to 2 mm.</li>
	<li>Apply the injury by activating impactor on the actuating device.</li>
	<li>Rotate the impact device out of the field and remove the animal from the stereotaxic frame.</li>
</ol>
5. Surgical site closure
<ol>
	<li>Control bleeding from the skull and injured cortical surface with direct pressure from a sterile cotton tipped applicator.</li>
	<li>Dry the skull with a sterile cotton tipped applicator.</li>
	<li>Close the scalp over the craniectomy using a commercially available surgical adhesive or monofilament suture. 
	NOTE: In this protocol a veterinary surgical adhesive is used to close the scalp. The bone flap is not replaced and is discarded.</li>
</ol>
6. Post-operative care and monitoring
<ol>
	<li>Administer post-operative analgesia (e.g., sustained release buprenorphine 0.1&#8211;0.5 mg/kg administered subcutaneously providing 72 h of sustained analgesia).</li>
	<li>Place the animal in the lateral decubitus recovery position in a clean pre-warmed cage.</li>
	<li>Observe the animals until awake and mobile, then return each mouse to its home cage.</li>
	<li>Ensure free access to food and water. Normal food and water intake typically resume within one to two hours after injury.</li>
	<li>Measure body weight every three days throughout the experiment.</li>
</ol>

<ol><li>Faul, M. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aFaul%2C+M+%22Traumatic+Brain+Injury+in+the+United+States%3A+Emergency+Department+Visits%2C+Hospitalizations+and+Deaths+2002-2006%22">Traumatic Brain Injury in the United States: Emergency Department Visits, Hospitalizations and Deaths 2002-2006</a>. , Centers for Disease Control and Prevention, National Center for Injury Prevention and Control. Atlanta (GA). (2010).</li>
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<li>Martin, L. A., Neighbors, H. W., Griffith, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+experience+of+symptoms+of+depression+in+men+vs+women%3A+analysis+of+the+National+Comorbidity+Survey+Replication%5BTitle%5D)+AND+%22JAMA+Psychiatry%22%5BJournal%5D)">The experience of symptoms of depression in men vs women: analysis of the National Comorbidity Survey Replication.</a> JAMA Psychiatry. 70 (10), 1100-1106 (2013).</li>
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<li>Belanger, H. G., Vanderploeg, R. D., McAllister, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Subconcussive+Blows+to+the+Head%3A+A+Formative+Review+of+Short-term+Clinical+Outcomes%5BTitle%5D)+AND+%22Journal+of+Head+Trauma+Rehabilitation%22%5BJournal%5D)">Subconcussive Blows to the Head: A Formative Review of Short-term Clinical Outcomes.</a> Journal of Head Trauma Rehabilitation. 31 (3), 159-166 (2016).</li>
<li>Carman, A. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Expert+consensus+document%3A+Mind+the+gaps-advancing+research+into+short-term+and+long-term+neuropsychological+outcomes+of+youth+sports-related+concussions%5BTitle%5D)+AND+%22Nature+Reviews+Neurology%22%5BJournal%5D)">Expert consensus document: Mind the gaps-advancing research into short-term and long-term neuropsychological outcomes of youth sports-related concussions.</a> Nature Reviews Neurology. 11 (4), 230-244 (2015).</li>
<li>Kramer, S. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+Contribution+to+the+Theory+of+Cerebral+Concussion%5BTitle%5D)+AND+%22Annals+of+Surgery%22%5BJournal%5D)">A Contribution to the Theory of Cerebral Concussion.</a> Annals of Surgery. 23 (2), 163-173 (1896).</li>
<li>Lighthall, J. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Controlled+cortical+impact%3A+a+new+experimental+brain+injury+model%5BTitle%5D)+AND+%22Journal+of+Neurotrauma%22%5BJournal%5D)">Controlled cortical impact: a new experimental brain injury model.</a> Journal of Neurotrauma. 5 (1), 1-15 (1988).</li>
<li>Dixon, C. E., Clifton, G. L., Lighthall, J. W., Yaghmai, A. A., Hayes, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+controlled+cortical+impact+model+of+traumatic+brain+injury+in+the+rat%5BTitle%5D)+AND+%22Journal+of+Neuroscience+Methods%22%5BJournal%5D)">A controlled cortical impact model of traumatic brain injury in the rat.</a> Journal of Neuroscience Methods. 39 (3), 253-262 (1991).</li>
<li>Schwulst, S. J., Trahanas, D. M., Saber, R., Perlman, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Traumatic+brain+injury-induced+alterations+in+peripheral+immunity%5BTitle%5D)+AND+%22Journal+of+Trauma+and+Acute+Care+Surgery%22%5BJournal%5D)">Traumatic brain injury-induced alterations in peripheral immunity.</a> Journal of Trauma and Acute Care Surgery. 75 (5), 780-788 (2013).</li>
<li>Trahanas, D. M., Cuda, C. M., Perlman, H., Schwulst, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Differential+Activation+of+Infiltrating+Monocyte-Derived+Cells+After+Mild+and+Severe+Traumatic+Brain+Injury%5BTitle%5D)+AND+%22Shock%22%5BJournal%5D)">Differential Activation of Infiltrating Monocyte-Derived Cells After Mild and Severe Traumatic Brain Injury.</a> Shock. 43 (3), 255-260 (2015).</li>
<li>Makinde, H. M., Cuda, C. M., Just, T. B., Perlman, H. R., Schwulst, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nonclassical+Monocytes+Mediate+Secondary+Injury%2C+Neurocognitive+Outcome%2C+and+Neutrophil+Infiltration+after+Traumatic+Brain+Injury%5BTitle%5D)+AND+%22Journal+of+Immunology%22%5BJournal%5D)">Nonclassical Monocytes Mediate Secondary Injury, Neurocognitive Outcome, and Neutrophil Infiltration after Traumatic Brain Injury.</a> Journal of Immunology. 199 (10), 3583-3591 (2017).</li>
<li>Thompson, H. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Lateral+fluid+percussion+brain+injury%3A+a+15-year+review+and+evaluation%5BTitle%5D)+AND+%22Journal+of+Neurotrauma%22%5BJournal%5D)">Lateral fluid percussion brain injury: a 15-year review and evaluation.</a> Journal of Neurotrauma. 22 (1), 42-75 (2005).</li>
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<li>Reneer, D. V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+multi-mode+shock+tube+for+investigation+of+blast-induced+traumatic+brain+injury%5BTitle%5D)+AND+%22Journal+of+Neurotrauma%22%5BJournal%5D)">A multi-mode shock tube for investigation of blast-induced traumatic brain injury.</a> Journal of Neurotrauma. 28 (1), 95-104 (2011).</li>
<li>Ma, X., Aravind, A., Pfister, B. J., Chandra, N., Haorah, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Animal+Models+of+Traumatic+Brain+Injury+and+Assessment+of+Injury+Severity%5BTitle%5D)+AND+%22Molecular+Neurobiology%22%5BJournal%5D)">Animal Models of Traumatic Brain Injury and Assessment of Injury Severity.</a> Molecular Neurobiology. , (2019).</li>
<li>Makinde, H. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Monocyte+depletion+attenuates+the+development+of+posttraumatic+hydrocephalus+and+preserves+white+matter+integrity+after+traumatic+brain+injury%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Monocyte depletion attenuates the development of posttraumatic hydrocephalus and preserves white matter integrity after traumatic brain injury.</a> PLoS One. 13 (11), e0202722(2018).</li>
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</ol>

The authors have no financial conflicts of interest.

There are several steps that are critical for applying a reliable and consistent injury. First, the mouse must reach a deep plane of surgical anesthesia ensuring no movement during the performance of the craniectomy. While numerous anesthetic regimens may be used to induce general anesthesia in rodents, anesthetics that induce respiratory depression such as inhalational anesthetics may result in respiratory arrest when combined with a severe TBI. This protocol utilizes ketamine (125 mg/kg) and xylazine (10 mg/kg) injecte...

The Centers for Disease Control and Injury Prevention estimate that approximately 2 million Americans sustain a traumatic brain injury (TBI) every year1,2. In fact, TBI contributes to over 30% of all injury related deaths in the United States with healthcare costs nearing $80 billion annually and almost $4 million per person per year surviving a severe TBI3,4,5. The impact of TBI is highlighted by the significant long-term neurocognitive and neuropsychiatric complications suffered by its survivors with the insidious onset of behavioral, cognitive, and motor impairments termed Chronic Traumatic Encephalopathy (CTE)6,7,8,9,10. Even subclinical concussive events&#8212;those impacts that do not result in clinical symptoms&#8212;can lead to long-term neurologic dysfunction11,12.
Animal models for the study of TBI have been employed since the late 1800&#8217;s13. In the 1980s, a pneumatic impactor for the purpose of modeling TBI was developed. This method is now referred to as controlled cortical impact (CCI)14. The control and reproducibility of CCI led researchers to adapt the model for use in rodents15. Our laboratory uses this model to induce TBI via a commercially available impactor and electronic actuating device16,17. This model is capable of producing a wide range of clinically applicable TBI states depending on the biomechanical parameters used. Histologic evaluation of TBI brains after a severe injury induced in our laboratory demonstrates significant ipsilateral cortical and hippocampal loss as well as contralateral edema and distortion. Additionally, CCI produces a consistent impairment in motor and cognitive function as measured by behavioral assays18. Limitations to CCI include the need for craniotomy and the expense of acquiring the impactor and actuating device.
Several additional models of TBI exist and are well established in the literature including the lateral fluid percussion model, weight drop model, and blast injury model19,20,21. While each of these models have their own distinct advantages their main drawbacks are mixed injury, high mortality and lack of standardization, respectively22. Furthermore, none of these models offer the accuracy, precision, and reproducibility of CCI. By adjusting the biomechanical parameters input into the actuating device, the CCI model allows the investigator precise control over size of the injury, depth of the injury, and kinetic energy applied to the brain. This gives investigators the ability to apply the entire spectrum of TBI to specific areas of the brain. It also permits the greatest reproducibility from experiment to experiment.

<table><tbody><tr><td>AnaSed Injection Xylazine Sterile Solution</td><td>LLOYD, Inc.</td><td>5939911020</td><td></td></tr><tr><td>Buprenorphine SR Lab 0.5mg/mL</td><td>Zoopharm-Wildlife Pharmaceuticals USA</td><td>BSRLAB0.5-182012</td><td></td></tr><tr><td>High Speed Rotary Micromotor KiT0</td><td>Foredom Electric Company</td><td>K.1070</td><td></td></tr><tr><td>Imapact one for Stereotaxix CCI</td><td>Leica Biosystems Nussloch GmbH</td><td>39463920</td><td></td></tr><tr><td>Ketathesia Ketamine HCl Injection USP</td><td>Henry Schein, Inc</td><td>56344</td><td></td></tr><tr><td>Mouse Specific Stereotaxic Base</td><td>Leica Biosystems Nussloch GmbH</td><td>39462980</td><td></td></tr><tr><td>Trephines for Micro Drill</td><td>Fine Science Tools, Inc</td><td>18004-50</td><td></td></tr></tbody></table>

murine model of controlled cortical impact for the induction of traumatic brain injury

Murine Model of Controlled Cortical Impact for the Induction of Traumatic Brain Injury

The Centers for Disease Control and Injury Prevention estimate that almost 2 million people sustain a traumatic brain injury (TBI) every year in the United States. In fact, TBI is a contributing factor to over a third of all injury-related mortality. Nonetheless, the cellular and molecular mechanisms underlying the pathophysiology of TBI are poorly understood. Thus, preclinical models of TBI capable of replicating the injury mechanisms pertinent to TBI in human patients are a critical research need. The controlled cortical impact (CCI) model of TBI utilizes a mechanical device to directly impact the exposed cortex. While no model can full recapitulate the disparate injury patterns and heterogeneous nature of TBI in human patients, CCI is capable of inducing a wide range of clinically applicable TBI. Furthermore, CCI is easily standardized allowing investigators to compare results across experiments as well as across investigative groups. The following protocol is a detailed description of applying a severe CCI with a commercially available impacting device in a murine model of TBI.

The impactor mounts directly on the stereotaxic frame allowing for as much as 10 µm resolution for control of the point of impact, depth and penetration. The electromagnetic forces employed can impart impact velocities ranging 1.5–6 m/s. This allows for unparalleled precision and reproducibility over the entire range of clinically relevant TBI. Investigators can run pilot experiments changing the injury parameters such as impactor tip size, impact velocity, and impact depth to determine the parameters that best produce t...

Here we describe a protocol for the induction of murine traumatic brain injury via an open-head controlled cortical impact.

The Centers for Disease Control and Injury Prevention estimate that almost 2 million people sustain a traumatic brain injury (TBI) every year in the ...

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This video demonstrates the procedure for inducing an open-skull traumatic brain injury in a mouse by making a midline skull incision, drilling through the skull and removing the bone flap to expose the brain cortex, and applying high pressure to induce trauma. The wound is then sutured to prepare the mouse model for subsequent experiments.

Generating a Traumatic Brain Injury Mouse Model Using an Impactor Probe

This video demonstrates the procedure for inducing traumatic brain injury (TBI) in a mouse model. An anesthetized mouse with an exposed skull is taken to create an opening in the skull, and then a controlled cortical injury is induced using an impact device. The injury damages the brain tissue, causing impaired cognitive function and motor coordination.

Generating a Mouse Model of Traumatic Brain Injury Using an Impactor Device

Source: Macheda, T., et. al. Electromagnetic Controlled Closed-Head Model of Mild Traumatic Brain Injury in Mice. J. Vis. Exp. (2022)This video demonstrates the procedure to induce a closed-head injury in a mouse model using a stereotaxic frame. The controlled impact replicates mild traumatic brain injury, allowing researchers to study neurological effects and recovery by measuring post-injury reflexes and behavior.

Induction of Mild Traumatic Brain Injury in a Mouse Model

Traumatic brain injuries (TBIs) remain a serious health problem. Using the controlled cortical impact surgery model, research on the effects of TBI and possible treatment methods may be performed.

Controlled Cortical Impact Model for Traumatic Brain Injury

Athletes absorb several hundred mild traumatic brain injuries (mTBI)/concussions every year; however, the consequence of these on the brain is poorly understood. Therefore, an animal model of single and repetitive mTBI that consistently replicates clinically relevant symptoms provides the means to advance the study of mTBI and concussion.

A Mouse Model of Single and Repetitive Mild Traumatic Brain Injury

This protocol demonstrates methodologies for a mouse model of open-skull traumatic brain injury and transplantation of cultured human induced pluripotent stem cell-derived cells into the injury site. Behavioral and histologic tests of outcomes from these procedures are also described in brief.

Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

Intraparenchymal hemorrhage and neuroinflammation accompanied by cerebral contusion can trigger severe secondary brain injury. This protocol details a mouse controlled cortical impact (CCI) model allowing researchers to study hemorrhage contusion and post-traumatic immune responses and explore potential therapeutics.

A Preclinical Controlled Cortical Impact Model for Traumatic Hemorrhage Contusion and Neuroinflammation

The protocol describes mild traumatic brain injury in a mouse model. In particular, a step-by-step protocol to induce a mild midline closed head injury and the characterization of the animal model is fully explained.

Electromagnetic Controlled Closed-Head Model of Mild Traumatic Brain Injury in Mice

Here, we present a protocol to establish a closed-head injury animal model replicating the neuroimage outcome of uncomplicated mild traumatic brain injury with the preserved brain structure in the acute phase and long-term brain atrophy. Longitudinal magnetic resonance imaging is the primary method used for evidence.

Development of an Uncomplicated Mild Traumatic Brain Injury Model Modified by Weight-Drop Method and Evidenced by Magnetic Resonance Imaging

Lateral fluid percussion (LFP), an established model of traumatic brain injury in mice, is demonstrated. LFP fulfills three major criteria for animal models: validity, reliability and clinical relevance. The procedure, consisting of surgical craniotomy, fixation of hub followed by induction of injury, resulting in focal and diffuse injuries, is described.

Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice

Learn controlled cortical impact for inducing traumatic brain injury in mice using this standardized protocol. This reproducible method enables behavioral, imaging, and molecular analyses of TBI mechanisms and recovery.

Murine Model of Controlled Cortical Impact for the Induction of Traumatic Brain Injury

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