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Chapters
- 00:04Title
- 00:38Induced Pluripotent Stem Cell (iPSC) Passaging and Maintenance
- 01:40Human Embryonic Stem Cell (hESC)-Qualified Matrix-Coated Plates and Coverslips
- 02:14Selected iPSC-Cardiomyocyte (CM) Plating
- 03:39Intracellular Calcium (Ca2+) Transient Assessment
- 05:42Results: Representative iPSC-CM Phenotypic Characterization, Differentiation Efficiency, and Calcium Amplitude Analyses
- 07:16Conclusion
Here we describe and validate a method to consistently generate robust human induced pluripotent stem cell-derived cardiomyocytes and characterize their function. These techniques may help in developing mechanistic insight into signaling pathways, provide a platform for large-scale drug screening, and reliably model cardiac diseases.
