Ventricular-like Cardiomyocytes
Ventricular-like Cardiomyocytes - Related Videos
11:33
Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes
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Cited by 15 •
2013
Kv channel dysfunction is associated with cardiac arrhythmias. In order to study the molecular mechanisms that lead to such arrhythmias we utilize a systematic protocol for isolation of atrial and ventricular cardiomyocytes from Kv channel ancillary subunit knockout mice. Isolated cardiomyocytes can then immediately be used for cellular electrophysiological studies, biochemical or immunofluorescence (IF) assays.
14:39
Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples
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Cited by 44 •
2014
Current knowledge on the cellular basis of cardiac diseases mostly relies on studies on animal models. Here we describe and validate a novel method to obtain single viable cardiomyocytes from small surgical samples of human ventricular myocardium. Human ventricular myocytes can be used for electrophysiological studies and drug testing.
11:00
Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes
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Cited by 18 •
2017
Intracellular calcium recycling plays a critical role in regulation of systolic and diastolic function in cardiomyocytes. Here, we describe a protocol to evaluate sarcoplasmic reticulum Ca2+ reserve and diastolic calcium removal function in cardiomyocytes by a calcium imaging system.
11:19
Isolation of Human Ventricular Cardiomyocytes from Vibratome-Cut Myocardial Slices
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Cited by 14 •
2020
Presented is a protocol for the isolation of human and animal ventricular cardiomyocytes from vibratome-cut myocardial slices. High yields of calcium-tolerant cells (up to 200 cells/mg) can be obtained from small amounts of tissue (<50 mg). The protocol is applicable to myocardium exposed to cold ischemia for up to 36 h.
12:10
Measuring Fast Calcium Fluxes in Cardiomyocytes
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Cited by 3 •
2011
We present a method to isolate rapid (microsecond) calcium events from slower fluxes in living cells using laser scanning confocal microscopy. The method measures fluorescence intensity fluctuations of calcium indicators by recording line scans of several hundred pixels in a cell. Histogram analysis allows us to isolate the time scales of different calcium fluxes.
FAQs
Ventricular-like cardiomyocytes reproduce ventricular-type electrical activity through a sequence of ion movements. Sodium-channel activity drives rapid depolarization, calcium entry contributes to the action potential and supplies the trigger for contraction, and potassium currents promote repolarization. The coordination of these phases allows investigators to examine how altered electrical behavior could affect ventricular function in experimental models.
Calcium handling links electrical excitation to mechanical contraction in these cells. When calcium enters during the action potential, its handling helps initiate the contractile response. Measuring calcium signals alongside contractility therefore provides complementary information: one readout reflects intracellular calcium behavior, while the other indicates the resulting mechanical output.
The main limitation is cellular maturity: ventricular-like cardiomyocytes resemble ventricular contractile cells but are not identical to mature adult ventricular cells. Consequently, electrical, calcium-handling, or contractility findings may not reproduce every feature of adult cardiac physiology. This distinction matters when researchers interpret disease models, drug responses, or regeneration studies and assess how broadly results may apply.
Researchers can produce these cells from pluripotent stem cells and then assess their behavior with complementary functional assays. Electrophysiology examines electrical activity, calcium imaging tracks calcium-related signals, and contractility assays evaluate mechanical performance. Using more than one readout helps connect action-potential behavior with calcium handling and contraction, rather than relying on a single aspect of cardiac function.
A useful interpretation compares electrical, calcium, and mechanical readouts as related but nonidentical aspects of function. Electrophysiology indicates how the action potential behaves, calcium imaging indicates whether calcium handling accompanies that activity, and contractility testing shows the mechanical consequence. Considering these measurements together allows researchers to identify which functional stage requires closer investigation.
They provide experimental systems for several medical research goals. Investigators can model cardiac disease, test whether compounds cause cardiotoxicity, support drug development, and explore cardiac regeneration. Their ventricular-like electrical and mechanical behavior makes the cells relevant to these questions, while differences from mature adult ventricular cells must be considered when translating findings to cardiac function.