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生成心室样HiPSC衍生的心肌细胞和用于钙处理特征的高质量细胞制剂
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JoVE Journal Medicine
Generation of Ventricular-Like HiPSC-Derived Cardiomyocytes and High-Quality Cell Preparations for Calcium Handling Characterization
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生成心室样HiPSC衍生的心肌细胞和用于钙处理特征的高质量细胞制剂

DOI:
10.3791/60135-v

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08:06 min

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January 17, 2020

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, , ,
1Cardiovascular Research Center,Icahn School of Medicine at Mount Sinai

Chapters

  • 00:04Title
  • 00:38Induced Pluripotent Stem Cell (iPSC) Passaging and Maintenance
  • 01:40Human Embryonic Stem Cell (hESC)-Qualified Matrix-Coated Plates and Coverslips
  • 02:14Selected iPSC-Cardiomyocyte (CM) Plating
  • 03:39Intracellular Calcium (Ca2+) Transient Assessment
  • 05:42Results: Representative iPSC-CM Phenotypic Characterization, Differentiation Efficiency, and Calcium Amplitude Analyses
  • 07:16Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述和验证一种持续产生强大的人类诱导多能干细胞衍生心肌细胞的方法，并描述其功能。这些技术可能有助于发展对信号通路的机械洞察力，为大规模药物筛查提供平台，并可靠地模拟心脏病。

Tags

Ventricular-like CardiomyocytesHiPSC-derived CardiomyocytesCalcium HandlingReproducibilityCell PassagingCardiac DifferentiationMetabolic SelectionDissociationSingle Cell SuspensionCell Concentration

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