Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Medicine

A subscription to JoVE is required to view this content.
You will only be able to see the first 20 seconds.

A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons
 

A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons

Article doi: 10.3791/60171
October 6th, 2019

Summary October 6th, 2019

Here, a step-by-step protocol for the preparation and cultivation of porcine split corneal buttons is presented. As this organo-typically cultivated organ culture model shows cell death rates within 15 days, comparable to human donor corneas, it represents the first model allowing long-term cultivation of non-human corneas without adding toxic dextran.

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter