Name: Stimulation Location Determination using a 3D Digitizer with High-Definition Transcranial Direct Current Stimulation (Video) | JoVE Journal
Uploaded: 2019-12-20T13:00:00.000+00:00
Duration: 7 min 20 s
Description: Learn to determine precise stimulation locations using a 3D digitizer with high-definition transcranial direct current stimulation. This method improves electrode placement accuracy and reproducibility for neuroscience research.

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5 minutes

This study was supported by the National Natural Science Foundation of China (31972906), Entrepreneurship and Innovation Program for Chongqing Overseas Returned Scholars (cx2017049), Fundamental Research Funds for Central Universities (SWU1809003), Open Research Fund of the Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences (KLMH2019K05), Research Innovation Projects of Graduate Student in Chongqing (CYS19117), and the Research Program Funds of the Collaborative Innovation Center of Assessment toward Basic Education Quality at Beijing Normal University (2016-06-014-BZK01, SCSM-2016A2-15003, and JCXQ-C-LA-1). We would like to thank Prof. Ofir Turel for his suggestions on the early draft of this manuscript.

The protocol meets the guidelines of the Institutional Review Board of Southwest University.
1. Determination of Stimulation Location
<ol>
	<li>Review the literature and confirm the stimulation location (here, the rTPJ)19,20,21.</li>
</ol>
2. Preparation of Electrode Holding Cap
NOTE: The following steps are shown in Figure 1.
<ol>
	<li>Ensure that all necessary materials are readily available: the 3D digitizer (Figure 2), standard measurement tape, a marking pen, the headform, and a swimming cap.</li>
	<li>Put the cap on the headform and mark the points on the cap.
	<ol>
		<li>Localize the Vertex (Cz). To do so, first mark the midpoint of the distance between the nasion and inion using a skin marker13,14,22. Then, measure the distance between the pre-auricular points and mark the midpoint. The point at which both points intersect is the Cz.</li>
		<li>Check the location of the center electrode and the return electrodes. Here, the stimulation was applied on rTPJ. The rTPJ roughly corresponds to the midpoint between CP6 and P6 in the 10-10 EEG system19,20,21.</li>
		<li>Find CP6 and P622,23,24,25. According to the proportional requirements of the 10-10 system, locate the approximate location of the rTPJ on the scalp and mark it on the cap.</li>
		<li>Adjust the radius of the four return electrodes based on the objectives11,14,26. After this decision, mark the center electrode and return electrode locations on the cap.</li>
	</ol>
	</li>
</ol>
3. 3D Digitizer Measurement
<ol>
	<li>Scan with the metal scanner to ensure that the environment for 3D digitizer is metal-free.</li>
	<li>Placement of the cap on the subject&#39;s head
	<ol>
		<li>Make sure that the references (Cz, Fpz, Oz, left preauricular point, and right preauricular point) on the cap align with the international 10-10 system for scalp location22. For example, localize the Vertex (Cz) on the scalp and place the cap onto the subject&#39;s head, aligning the cap&#39;s Cz to the subjects.</li>
	</ol>
	</li>
	<li>Arranging the 3D digitizer equipment
	<ol>
		<li>Connect the 3D digitizer to the computer using the Universal Serial Bus (USB) interface and make sure that the digitizer software is available and ready27.</li>
		<li>Put the source in front of the subject and fasten the elastic rope of the sensor around the head. Importantly, make sure that neither the source nor sensor moves during 3D digitizer measurement. 
		NOTE: The source is a magnetic transmitter that emits an electromagnetic dipole field. The sensor is a receiver that detects the field.</li>
		<li>Open the digitizer software on the computer and make sure that the 3D digitizer system communicates with the software.</li>
		<li>Test the accuracy of the stylus. Find a length of 10 cm on the ruler and record the zero graduation and ten graduation, respectively, using the stylus. 
		NOTE: The measurement distance between the two recording points of the 3D digitizer should be captured. Compare the error with the reading from the 3D tracker.</li>
		<li>Select the New icon and create a new subject file. Select the Sessions box, then Reference. 
		NOTE: Using the 3D digitizer stylus, the reference position data (Cz, inion, nasion, left ear, right ear) of the subject are collected according to the software prompts.</li>
		<li>To cater to the requirement of fNIRS experiments, use the Transmitter, Detector, and Channel options. Collect the position data of the center electrode and four return electrodes 3x for the transmitter, detector, and channel, in order to reduce error. Ensure that five electrodes are numbered and localize in turn.</li>
		<li>Save the three files that are generated.</li>
	</ol>
	</li>
</ol>
4. Data Conversion and Spatial Registration
<ol>
	<li>Select the three files into the NIRS-SPM to achieve the real coordinates registration into MNI space28. Affine transform the reference points and five electrode points in participants to the corresponding points in each entry according to the MRI database in MNI space.</li>
	<li>Register the data to anatomical automatic labels and Brodmann areas and register the spatial information of the five electrode points to both of these.</li>
	<li>Compare the coordinates of stimulation in previous research with the obtained coordinates20,29.</li>
	<li>Make a small cut aligned to the five points marked on the cap, such that the plastic casing is embedded snugly into the cap.</li>
</ol>
5. Stimulation
<ol>
	<li>Make sure that participant have no contraindications (i.e., history of neurological or psychiatric disorders) for HD-tDCS1,3 and that they provided written informed consent prior to the study (including HD-tDCS stimulation).</li>
	<li>For device installation, ensure that all the necessary materials are available (Figure 3). Install the device as detailed in published literature14. A brief description is provided below.
	<ol>
		<li>Install the batteries and check that they are charged.</li>
		<li>Connect the conventional tDCS and 4x1 Stimulation Adapter.</li>
		<li>Connect the cables of five Ag/AgCI sintered ring electrodes to the matching receivers on the 4x1 adapter output cable.</li>
		<li>Check that all materials are connected correctly.</li>
	</ol>
	</li>
	<li>Measure the head of the participant and place the cap on the head.
	<ol>
		<li>Embed the five plastic HD casings in the swimming cap.</li>
		<li>Localize the Cz, Fpz, and Oz of the subject13,14. Adjust the reference on the cap to align with the international 10-10 system for scalp locations22. Once the cap is in position, ensure that it does not move.</li>
		<li>Collect the position data of the stimulated brain areas using the 3D digitizer. Make the corresponding adjustments according to the generated data.</li>
	</ol>
	</li>
	<li>Cover the scalp surface with electrically conductive gel. First, carefully separate the hair through the opening of the plastic casing using the end of a plastic syringe, until the scalp is exposed. Then, cover the exposed scalp with the electrically conductive gel through the plastic casing opening on the scalp surface.</li>
	<li>Set the parameters of the tDCS device: quality value, stimulus duration, intensity, and condition setting.
	<ol>
		<li>Turn on the 4x1 Multichannel Stimulation Adapter.</li>
		<li>Ensure that the default setting is SCAN, which shows the impedance of one electrode at a time in the display window by scanning the electrodes14,30,31. Here, the impedance is described as &#34;quality value&#34;. Values below 1.5 indicate sufficient quality14,30,31. In this case, the values were lower than 1. 
		NOTE: If the impedance value exceeds these required limits, open the cap of the plastic casing with high impedance and adjust the hair and electrode to obtain the desired impedance value.</li>
		<li>Press the &#34;MODE SELECT&#34; button and switch from &#34;SCAN&#34; to &#34;PASS&#34;, after the impedance values are acceptable.</li>
		<li>Select the center-anode or center-cathode by pressing the &#34;POLARITY&#34; button. &#34;CENTRAL ANODE&#34; is the default setting.</li>
		<li>Adjust the settings on the conventional tDCS device to include stimulus duration (min), intensity (mA) and sham condition setting. In this case, anodal active stimulation was 1.5 mA, and the stimulus lasted 20 min. Next, push the &#34;RELAX&#34; lever to switch to full current.</li>
		<li>Once everything is set, initiate the stimulation. Press the &#34;START&#34; button, and the DC intensity will ramp up until the target current is reached. The timer will then show the remaining time. 
		NOTE: Some participants may feel uncomfortable during periods of increased DC intensity. In such cases, the current may be moderately decreased slightly for a few seconds by pulling down the &#34;RELAX&#34; lever. Then, push the dolly bar to full current, gradually, when participants feel comfortable again.</li>
	</ol>
	</li>
</ol>
6. Post-stimulation
<ol>
	<li>When the stimulation is over, turn the lever slowly to adjust the current to zero before turning off the power. Otherwise, participants may perceive stinging sensation or dizziness when turning off the power directly.</li>
	<li>After the stimulation, open the plastic cap and remove the Ag/AgCI sintered ring electrodes from the casing.</li>
	<li>Remove the swimming cap and clean the materials. Provide participants with tools to clean their hair.</li>
	<li>Ask participants to fill out a questionnaire after each stimulation session, if necessary (e.g., to measure adverse effects of screening following HD-tDCS, participant tolerance to brain stimulation, etc.; see Supplementary File).</li>
</ol>

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</ol>

The authors have nothing to disclose.

Compared to traditional tDCS, HD-tDCS increases the focality of stimulation. Typical sites of stimulation are often based on the 10-20 EEG system. However, determining the precise stimulation points beyond this system can be difficult. This paper combines a 3D digitizer with HD-tDCS to determine stimulation points beyond the 10-20 system. It is important to clearly define the steps and precautions for making and using the electrode cap in such cases.
In general, the position of target stimulat...

Transcranial direct current stimulation (tDCS) is a noninvasive technique that modulates cortical excitability with weak direct currents over the scalp. It aims to establish causality between neural excitability and behavior in healthy humans1,2,3. In addition, as a motor neurorehabilitation tool, tDCS is widely used in the treatment of Parkinson's disease, stroke, and cerebral palsy4. Existing evidence suggests that traditional pad-based tDCS produces current flow through a relatively larger brain region5,6,7. High-definition transcranial direct current stimulation (HD-tDCS), with the center ring electrode sitting over a target cortical region surrounded by four return electrodes8,9, increases focality by circumscribing four ring areas5,10. In addition, changes in excitability of the brain induced by HD-tDCS have significantly larger magnitudes and longer durations than those generated by traditional tDCS7,11. Therefore, HD-tDCS is widely used in research7,11.
Noninvasive brain stimulation (NIBS) requires specialized methods to ensure that a stimulation site is present in the standard MNI and Talairach systems12. Neuronavigation is a technique that allows for mapping interactions between transcranial stimuli and the human brain. Its visualization and 3D image data are used for precise stimulation. In both tDCS and HD-tDCS, a common assessment of stimulation sites on the scalp is typically the EEG 10-20 system13,14. This measurement is widely used for placing the tDCS pads and optode holders for functional near infrared spectroscopy (fNIRS) in the initial stage13,14,15.
Determining the precise stimulation points when using the 10-20 system can be difficult (e.g., in the temporo-parietal junction [TPJ]). The best way to solve this is to obtain structural images from participants using magnetic resonance imaging (MRI), then obtain the exact probe position by matching target points to their structural images using digitizing products15. MRI provides good spatial resolution but is expensive to use15,16,17. Moreover, some participants (e.g., those with metal implants, claustrophobic people, pregnant women, etc.) cannot be subjected to MRI scanners. Therefore, there is a strong need for a convenient and efficient way to overcome the abovementioned limitations and increase accuracy in determining stimulation points.
This protocol uses a 3D digitizer to overcome these limitations. Compared to MRI, key advantages of a 3D digitizer are low costs, simple application, and portability. It combines five reference points (i.e., Cz, Fpz, Oz, left preauricular point, and right preauricular point) of individuals with location information of the target stimulation points. Then, it produces a 3D position of electrodes on the subject's head and estimates their cortical positions by fitting with the vast data from the structural image12,15. This probabilistic registration method enables the presentation of transcranial mapping data in the MNI coordinate system without recording a subject's magnetic resonance images. The approach generates anatomical automatic labels and Brodmann areas11.
The 3D digitizer, used to mark space coordinates based on the data from structural images, was first used to determine the position of optodes in fNIRS research18. For those who use HD-tDCS, a 3D digitizer breaks the finite stimulation points of the EEG 10-20 system. The distance of the four return electrodes and center electrode is flexible and can be adjusted as needed. When using the 3D digitizer with this protocol, the coordinates of the rTPJ were obtained, which is beyond the 10-20 system. Also shown are the procedures for targeting and stimulating the right temporo-parietal junction (rTPJ) of the human brain.

<table><tbody><tr><td>1X1 Low Intensity transcranial DC Stimulator</td><td>Soterix Medical</td><td>1300A</td><td></td></tr><tr><td>3-dimensional Polhemus-Patriot Digitizer</td><td>POLHEMUS</td><td>1A0453-001</td><td>PATRIOT system component</td></tr><tr><td>4X1 Multi-Channel Stimulation Interface</td><td>Soterix Medical</td><td>4X1-C3</td><td></td></tr><tr><td>Dell desktop computer</td><td>Dell</td><td>CRFC4J2</td><td>Master computer to run 3D digitizer application</td></tr></tbody></table>

stimulation location determination using a 3d digitizer with high-definition transcranial direct current stimulation

Stimulation Location Determination using a 3D Digitizer with High-Definition Transcranial Direct Current Stimulation

The abundance of neuroimaging data and rapid development of machine learning has made it possible to investigate brain activation patterns. However, causal evidence of brain area activation leading to a behavior is often left missing. Transcranial direct current stimulation (tDCS), which can temporarily alter brain cortical excitability and activity, is a noninvasive neurophysiological tool used to study causal relationships in the human brain. High-definition transcranial direct current stimulation (HD-tDCS) is a noninvasive brain stimulation (NIBS) technique that produces a more focal current compared to conventional tDCS. Traditionally, the stimulation location has been roughly determined through the 10-20 EEG system, because determining precise stimulation points can be difficult. This protocol uses a 3D digitizer with HD-tDCS to increase accuracy in determination of stimulation points. The method is demonstrated using a 3D digitizer for more accurate localization of stimulation points in the right temporo-parietal junction (rTPJ).

Using the methods presented, coordinates of the rTPJ were determined, which requires stimulation points beyond the 10-20 system. First, the circumference of the headform should be similar to the actual head. Here, the length of the nasion to inion of the headform was ~36 cm, and the length between the bilateral preauricular was ~37 cm.
The steps for producing the electrode cap guide the measuring positions of the 10-20 system. Here, Nz, Iz, Cz, Fpz, Oz, Pz, T8, T7, C4, P8, O2, P4, C6, P6, and ...

Presented here is a protocol to achieve higher accuracy in determination of stimulation location combining a 3D digitizer with high-definition transcranial direct current stimulation.

The abundance of neuroimaging data and rapid development of machine learning has made it possible to investigate brain activation patterns. However, ...

stimulation-location-determination-using-3d-digitizer-with-high

Research

JoVE Journal

Neuroscience

Transcranial direct current stimulation (tDCS) is an established technique to modulate cortical excitability1,2. It has been used as an investigative tool in neuroscience due to its effects on cortical plasticity, easy operation, and safe profile. One area that tDCS has been showing encouraging results is pain alleviation 3-5.

Electrode Positioning and Montage in Transcranial Direct Current Stimulation

Source: Chen, W., et al. Stimulation Location Determination using a 3D Digitizer with High-Definition Transcranial Direct Current Stimulation. J. Vis. Exp. (2019)This video demonstrates the procedure for delivering high-definition transcranial direct current stimulation (HD-tDCS) by accurately positioning electrodes using the 10–10 system, mapping their locations with a 3D digitizer, and applying stimulation to modulate neuronal activity in a targeted brain region.

High-Definition Transcranial Direct Current Stimulation for Activation of the Target Brain Region

High-definition transcranial direct current stimulation (HD-tDCS), with its 4x1-ring montage, is a noninvasive brain stimulation technique that combines both the neuromodulatory effects of conventional tDCS with increased focality. This article provides a systematic demonstration of the use of 4x1 HD-tDCS, and the considerations needed for safe and effective stimulation.

Technique and Considerations in the Use of 4x1 Ring High-definition Transcranial Direct Current Stimulation (HD-tDCS)

Transcranial direct current stimulation (tDCS) is a non-invasive brain stimulation technique that has shown initial therapeutic effects in several neurological conditions. The main mechanism underlying these therapeutic effects is the modulation of cortical excitability. Therefore, online monitoring of cortical excitability would help guide stimulation parameters and optimize its therapeutic effects. In the present article we review the use of a novel device that combines simultaneous tDCS and...

Simultaneous EEG Monitoring During Transcranial Direct Current Stimulation

Transcranial direct current stimulation (tDCS) is a noninvasive brain stimulation technique. It has successfully been used in basic research and clinical settings to modulate brain function in humans. This article describes the implementation of tDCS and simultaneous functional magnetic resonance imaging (fMRI), to investigate the neural basis of tDCS effects.

Transcranial Direct Current Stimulation and Simultaneous  Functional Magnetic Resonance Imaging

The combined use of transcranial direct current stimulation and robotic therapy as an add-on for conventional rehabilitation therapy may result in improved therapeutic outcomes due to modulation of brain plasticity. In this article, we describe the combined methods used in our institute for improving motor performance after stroke.

The Combined Use of Transcranial Direct Current Stimulation and Robotic Therapy for the Upper Limb

This protocol describes the method of neuronavigated electrode placement for focal, transcranial direct current stimulation (tDCS) administered during functional magnetic resonance imaging (fMRI).

Neuronavigated Focalized Transcranial Direct Current Stimulation Administered During Functional Magnetic Resonance Imaging

This study presents a novel transcranial direct current stimulation (tDCS) protocol combined with cognitive stimulation to address post-stroke hemispatial neglect. Initial data from a pilot patient ensure the procedure's feasibility and suggest potential efficacy, providing a foundation for a future parallel, triple-blind, controlled clinical trial.

Randomized, Triple-Blind, and Parallel-Controlled Trial of Transcranial Direct Current Stimulation for Cognitive Rehabilitation after Stroke

This study proposed an experimental paradigm that applies High-Definition Transcranial Direct Current Stimulation (HD-tDCS) to improve human metacognition in human-AI delegation tasks. The results demonstrated the effectiveness of HD-tDCS in enhancing metacognitive sensitivity.

High-definition Transcranial Direct Current Stimulation over Right Dorsolateral Prefrontal Cortex to Enhance Metacognitive Sensitivity

This protocol describes high-definition transcranial direct current stimulation during sleep with simultaneous electroencephalography monitoring to investigate causal relationships between targeted brain regions and sleep as well as its related processes.

High-Definition Transcranial Direct Current Stimulation During Sleep

This protocol introduces a scalp acupuncture-based method for localizing the primary motor cortex (M1) hotspot for the flexor digitorum superficialis (FDS) muscle and validates its effectiveness against the conventional C3 site.

A Novel Scalp Acupuncture-based Method to Target the Hand Motor Hotspot for Non-invasive Brain Stimulation

Learn to determine precise stimulation locations using a 3D digitizer with high-definition transcranial direct current stimulation. This method improves electrode placement accuracy and reproducibility for neuroscience research.

Stimulation Location Determination using a 3D Digitizer with High-Definition Transcranial Direct Current Stimulation

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