Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

This protocol is significant because it allows scientists to perform straightforward genetic analysis of bacterial wilt disease in tomato. The main advantage of this technique is that the manipulation of gene expression and subsequent assays can be performed in a short time and with the small requirements of equipment and plant growth space. This is method is very versatile, and it may be applied to other crop plants to perform both pathogen inoculation and other physiological assays.

To maintain the sterility during manipulation of seedlings in vitro, it is important to keep the plates closed when they are outside of the flow hood. This is an easy technique, but it requires multiple step for manipulation. The visualization of this method will help to see small tricks that are difficult to explain in the manuscript, helping other researchers to implement the method in their labs.