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JoVE Journal
Bioengineering

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Tissue-Engineered Graft for Circumferential Esophageal Reconstruction in Rats
 

Tissue-Engineered Graft for Circumferential Esophageal Reconstruction in Rats

Article doi: 10.3791/60349
February 10th, 2020

Summary February 10th, 2020

Esophageal reconstruction is a challenging procedure, and development of a tissue-engineered esophagus that enables regeneration of esophageal mucosa and muscle and that can be implanted as an artificial graft is necessary. Here, we present our protocol to generate an artificial esophagus, including scaffold manufacturing, bioreactor cultivation, and various surgical techniques.

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