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Tissue-Engineered Graft for Circumferential Esophageal Reconstruction in Rats
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Bioengineering
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JoVE Journal Bioengineering
Tissue-Engineered Graft for Circumferential Esophageal Reconstruction in Rats

Tissue-Engineered Graft for Circumferential Esophageal Reconstruction in Rats

DOI:
10.3791/60349-v

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08:56 min

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February 10, 2020

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, , , , ,
1Department of Otorhinolaryngology-Head and Neck Surgery,Seoul National University, College of Medicine, 2Department of Biomedical Engineering,Inje University, Gimhae, 3Department of Nature-Inspired Nanoconvergence Systems,Korea Institute of Machinery and Materials

Chapters

  • 00:04Introduction
  • 01:27Cell Seeding and Bioreactor Cultivation
  • 02:51Surgical Preparation
  • 03:20Gastrostomy Surgery
  • 04:30Esophageal Transplantation
  • 06:18Results: Representative Esophageal Transplantation and Reconstructed Esophagus Histological Analyses
  • 08:02Conclusion

Summary

Automatic Translation

Automatic Translation

Esophageal reconstruction is a challenging procedure, and development of a tissue-engineered esophagus that enables regeneration of esophageal mucosa and muscle and that can be implanted as an artificial graft is necessary. Here, we present our protocol to generate an artificial esophagus, including scaffold manufacturing, bioreactor cultivation, and various surgical techniques.

Tags

Circumferential Esophageal ReconstructionRat ModelTwo-layered Tubular ScaffoldNanofiber3D-printingMicrovascular AnastomosisBioreactor CultivationAdipose-derived Mesenchymal Stem CellsLIVE/DEAD AssayT-tube PlacementEsophageal Repair

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