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Neuroscience

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JoVE Journal Neuroscience
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Microinjectrode System for Combined Drug Infusion and Electrophysiology

Article DOI: 10.3791/60365-v • 08:30 min • November 13th, 2019
November 13th, 2019 •
1, 1,2, 3,4, 1
1Department of Ophthalmology and Visual Sciences, University of Utah, 2Department of Biomedical Engineering, University of Utah, 3Department of Electrical Engineering and Computer Science, University of California, Irvine, 4Department of Biomedical Engineering, University of California, Irvine

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Summary
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We present a microinjectrode system designed for electrophysiology and assisted delivery of experimental probes (i.e., nanosensors, microelectrodes), with optional drug infusion. Widely available microfluidic components are coupled to a cannula containing the probe. A step-by-step protocol for microinjectrode construction is included, with results during muscimol infusion in macaque cortex.

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Electrophysiology Experimental Probes Microelectrodes Nanosensors Awake Behaving Animals Cannula Dura Mater Microfluidic Infusion Nanoliter Scale Probe Insertion T Junction Ferrule Tubing
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