Nanoliter Delivery
Nanoliter Delivery - Related Videos
09:32
LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations
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Cited by 66 •
2013
Ice binding proteins (IBPs), also known as antifreeze proteins, inhibit ice growth and are a promising additive for use in the cryopreservation of tissues. The main tool used to investigate IBPs is the nanoliter osmometer. We developed a home-designed cooling stage mounted on an optical microscope and controlled using a custom-built LabVIEW routine. The nanoliter osmometer described here manipulated the sample temperature in an ultra-sensitive manner.
05:06
Tumor Allotransplantation in Drosophila melanogaster with a Programmable Auto-Nanoliter Injector
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Cited by 10 •
2021
This protocol provides detailed guidance for the initial and continued generational allotransplantation of Drosophila tumors into the abdomen of adult hosts for studying various aspects of neoplasia. Using an autoinjector apparatus, researchers can achieve improved efficiency and tumor yields compared to those achieved by traditional, manual methods.
10:34
Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation
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Cited by 12 •
2010
This protocol describes a non-viral method of delivery of genetic constructs to a certain area of living rodent brain. The method consists of plasmid preparation, micropipette fabrication, neonatal rat pup surgery, microinjection of the construct, and in vivo electroporation.
03:21
Localized Delivery of Bacteria into the Intestinal Lumen of a Zebrafish Larva Using Microinjection
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2025
Source: Li, J. et al. Development of a Larval Zebrafish Infection Model for Clostridioides difficile. J. Vis. Exp. (2020)This video demonstrates the microinjection-based delivery of fluorescently labeled Clostridioides difficile into the intestinal lumen of an anesthetized zebrafish larva. It outlines the steps involved in larval preparation, bacterial injection, and fluorescence microscopy–based confirmation of bacterial localization within the intestine.
04:17
Electrophoretic Delivery of GABA into the Epileptic Focus of an Anesthetized Mouse
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2025
Source: Slezia, A. et al. Electrophoretic Delivery of γ-aminobutyric Acid (GABA) into Epileptic Focus Prevents Seizures in Mice. J. Vis. Exp. (2019)This video demonstrates the electrophoretic delivery of gamma-aminobutyric acid (GABA) to inhibit seizure activity in an anesthetized mouse. It outlines the steps involved in probe implantation, neural activity recording, seizure induction, and controlled GABA delivery via a microfluidic ion pump to assess its therapeutic efficacy.
FAQs
These control modes regulate how liquid moves through a fine capillary or microscale dispenser. Pressure can drive movement, displacement can deliver a defined volume, and flow control can govern the rate or amount reaching the target. Using a calibrated control scheme helps convert the dispensing setting into a more predictable dose, which is important when neural experiments use very little material.
Spatial accuracy matters because the intended effect may depend on placing a reagent at a defined neural location rather than allowing it to spread broadly. Nanoliter delivery supports this localization while limiting diffusion and tissue disruption. That combination helps distinguish responses associated with the selected site from effects caused by wider exposure or unnecessary disturbance.
By dispensing only a billionth-of-a-liter-scale volume, the approach reduces sample consumption when reagents are scarce. Dose control also makes limited stocks more useful across localized administrations, whether the material is a drug, tracer, genetic material, or another reagent. This is especially relevant to experiments where each target receives a carefully constrained quantity rather than a broad excess.
A basic workflow begins by selecting a fine capillary or microscale dispenser, placing the reagent in that delivery system, and establishing calibrated pressure, displacement, or flow control. The operator then positions the outlet at the defined target and dispenses the selected volume. The result should be localized placement with minimal diffusion and tissue disruption, as required by the experiment.
Nanoliter delivery can be applied to neural tissue, cultured cells, and model organisms. Depending on the study, the delivered material may be a drug, tracer, genetic material, or other reagent. This flexibility allows the same volume-control principle to support localized pharmacological treatment, labeling, or molecular manipulation in different experimental settings.
In neuroscience, the method is useful when researchers need to examine neural circuits, cellular responses, or brain disease mechanisms at a localized site. Precise placement and reduced sample use can support experiments that compare responses to targeted exposure while conserving material. The resulting control strengthens interpretation of how a reagent affects tissue, cells, or model-organism nervous systems.