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Microinjectrode System for Combined Drug Infusion and Electrophysiology
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Microinjectrode System for Combined Drug Infusion and Electrophysiology

Microinjectrode System for Combined Drug Infusion and Electrophysiology

DOI:
10.3791/60365-v

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08:30 min

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November 13, 2019

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, , ,
1Department of Ophthalmology and Visual Sciences,University of Utah, 2Department of Biomedical Engineering,University of Utah, 3Department of Electrical Engineering and Computer Science,University of California, Irvine, 4Department of Biomedical Engineering,University of California, Irvine

Chapters

  • 00:04Title
  • 00:46Construction of the Microinjectrode for Stimulation and Recording
  • 02:21Microfluidic System
  • 03:22Preparation of the Microfluidic System
  • 05:46Performing Recording for an Infusion Experiment
  • 06:38Results: Memory Guided Saccade Task during Muscimol Infusion in FEF (Frontal Eye Field)
  • 07:26Conclusion

Summary

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We present a microinjectrode system designed for electrophysiology and assisted delivery of experimental probes (i.e., nanosensors, microelectrodes), with optional drug infusion. Widely available microfluidic components are coupled to a cannula containing the probe. A step-by-step protocol for microinjectrode construction is included, with results during muscimol infusion in macaque cortex.

Tags

Keywords: Microinjectrode SystemDrug InfusionElectrophysiologyMicroelectrodesNanosensorsAwake Behaving AnimalsMicrofluidic InfusionNanoliter ScaleCannulaT JunctionCapillary TubingGold PinEpoxy GlueThree-way ValvesMicrofluidic Circuit

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