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Chemical-Induced Skin Carcinogenesis Model Using Dimethylbenz[a]Anthracene and 12-O-Tetradecanoyl Phorbol-13-Acetate (DMBA-TPA)
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JoVE Journal Cancer Research
Chemical-Induced Skin Carcinogenesis Model Using Dimethylbenz[a]Anthracene and 12-O-Tetradecanoyl Phorbol-13-Acetate (DMBA-TPA)

Chemical-Induced Skin Carcinogenesis Model Using Dimethylbenz[a]Anthracene and 12-O-Tetradecanoyl Phorbol-13-Acetate (DMBA-TPA)

DOI:
10.3791/60445-v

•

04:12 min

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December 19, 2019

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, , , ,
1Faculty of Medicine and Health Technology,Tampere University and Tampere University Hospital, 2Department of Clinical Microbiology,Fimlab Laboratories

Chapters

  • 00:05Title
  • 00:56Skin Papilloma Induction and Promotion
  • 02:00Sample Collection
  • 02:43Results: Representative Outcomes and Histological Analyses in the DMBA-TPA Model
  • 03:12Conclusion

Summary

Automatic Translation

Automatic Translation

Two-stage skin carcinogenesis is induced by two topically applied chemicals. A mutagen 7,12-dimethylbenz[a]anthracene) causes mutations in the epidermal cells and a continuous application of general growth stimulator 12-O-tetradecanoyl phorbol-13-acetate accelerates skin papilloma formation.

Tags

Keyword Extraction:Chemical-induced Skin CarcinogenesisDMBA-TPATumor InitiationTumor ProgressionSquamous Cell CarcinomaImmune SystemAngiogenesisDMBATPAPapillomaSkinImmunohistochemical AnalysisGene ExpressionProtein AnalysisFlow Cytometric Analysis

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