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Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures (MAAC)
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Biology
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Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures (MAAC)

Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures (MAAC)

DOI:
10.3791/60485-v

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06:28 min

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February 13, 2020

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, ,
1Bioscience Metabolism, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D,AstraZeneca, 2The Lundberg Laboratory for Diabetes Research,University of Gothenburg, 3Wallenberg Centre for Molecular and Translational Medicine,University of Gothenburg

Chapters

  • 00:05Introduction
  • 00:53Human Subcutaneous Adipose Tissue Dissection
  • 01:32Collagenase Treatment
  • 02:21Mature Adipocyte Purification
  • 03:20Mature Adipocyte Packing
  • 03:51Mature Adipocyte Seeding and Maintenance
  • 04:50Results: Representative Mature Adipocyte Aggregate Culture (MAAC) Appearance and Response to Diverse Pharmacological Agonism
  • 05:55Conclusion

Summary

Automatic Translation

Automatic Translation

Membrane mature adipocyte aggregate culture (MAAC) is a new method to culture mature human adipocytes. Here we detail how to isolate adipocytes from human adipose and how to set up MAAC.

Tags

Keywords: Mature AdipocyteAdipocyte CultureMembrane Mature Adipocyte Aggregate Cultures (MAAC)Adipose BiologyDiabetesObesityCell InteractionsAdipocyte IsolationAdipocyte SuspensionAdipocyte WashAdipocyte Separation

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