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Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures (MAAC)
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Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures (MAAC)

Article DOI: 10.3791/60485-v • 06:28 min • February 13th, 2020
February 13th, 2020 •
1, 1, 1,2,3
1Bioscience Metabolism, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca, 2The Lundberg Laboratory for Diabetes Research, University of Gothenburg, 3Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg

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Summary
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Membrane mature adipocyte aggregate culture (MAAC) is a new method to culture mature human adipocytes. Here we detail how to isolate adipocytes from human adipose and how to set up MAAC.

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Isolation Culture Human Mature Adipocytes Membrane Mature Adipocyte Aggregate Cultures MAAC Adipose Biology Long Term Adipocyte Adipocyte Culture Technique Cell Interactions New Treatments Diabetes Obesity Protocol Gene Quality Viability Subcutaneous Adipose Tissue Sample Medium 199 Fibrotic Vessels Scissors Release Adipocytes Trimmed Fat Cell Release Smooth Homogenou
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