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Journal / Immunology and Infection / 使用IBA1和TMEM119进行小鼠脑微胶质密度、形态学和周围骨髓细胞渗透分析的免疫荧光染色…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
使用IBA1和TMEM119进行小鼠脑微胶质密度、形态学和周围骨髓细胞渗透分析的免疫荧光染色
Non-Invasive Model of Neuropathogenic Escherichia coli Infection in the Neonatal Rat
Journal (Immunology and Infection)
Non-Invasive Model of Neuropathogenic Escherichia coli Infection in the Neonatal Rat
Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans
Journal (Immunology and Infection)
Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans
 
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使用IBA1和TMEM119进行小鼠脑微胶质密度、形态学和周围骨髓细胞渗透分析的免疫荧光染色

Article DOI: 10.3791/60510-v • 10:40 min • October 27th, 2019
October 27th, 2019 •
1,2, 1,2, 1,3, 1,2,4, 1,2,4, 1,2
1Axe Neurosciences, Centre de Recherche du CHU de Québec-Université Laval, 2Département de Médecine Moléculaire, Faculté de Médecine, Université Laval, 3Integrated Program in Neuroscience, McGill University, 4Center for Brain Immunology and Glia (BIG), University of Virginia

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该协议描述了IBA1和TMEM119免疫荧光结化的分步工作流程，除了分析微胶质密度、分布和形态，以及小鼠脑组织中外周髓细胞渗透。

Tags

免疫学和感染， 问题 152， 小鼠， 大脑， 微胶质， 骨髓细胞， 神经免疫学， 免疫荧光， IBA1， TMEM119， 显微镜
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