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Journal / Immunology and Infection / Immunofluorescence farging bruke IBA1 og TMEM119…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Immunofluorescence farging bruke IBA1 og TMEM119 for Mikrogliaaggregater Density, morfologi og perifere myelogen Cell infiltrasjon analyse i Mouse Brain
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Immunofluorescence farging bruke IBA1 og TMEM119 for Mikrogliaaggregater Density, morfologi og perifere myelogen Cell infiltrasjon analyse i Mouse Brain

Article DOI: 10.3791/60510-v • 10:40 min • October 27th, 2019
October 27th, 2019 •
1,2, 1,2, 1,3, 1,2,4, 1,2,4, 1,2
1Axe Neurosciences, Centre de Recherche du CHU de Québec-Université Laval, 2Département de Médecine Moléculaire, Faculté de Médecine, Université Laval, 3Integrated Program in Neuroscience, McGill University, 4Center for Brain Immunology and Glia (BIG), University of Virginia

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Denne protokollen beskriver en trinnvis arbeidsflyt for immunofluorescent costaining av IBA1 og TMEM119, i tillegg til analyse av mikrogliaaggregater tetthet, distribusjon og morfologi, samt perifere myelogen celle infiltrasjon i mus hjernevev.

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Immunologi og infeksjon mus hjerne mikroglia myelogen celler neuroimmunology immunofluorescence IBA1 TMEM119 mikroskopi
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