Name: A Revised Surgical Approach to Induce Endolymphatic Hydrops in the Guinea Pig (Video) | JoVE Journal
Uploaded: 2020-06-04T13:30:00.000+00:00
Duration: 6 min 5 s
Description: Learn a revised surgical method to induce endolymphatic hydrops in guinea pigs using an extradural approach. This technique enables rapid induction with minimal risk for studying cochlear function and inner ear disorders.

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5 minutes

We thank Shannon M. Lefler for assistance with figures and the Table of Materials. Research reported in this publication was supported by the National Institute of Deafness and Other Communication Disorders within the National Institutes of Health, through the &#34;Development of Clinician/Researchers in Academic ENT&#34; training grant, award number T32DC000022 (C.V.V.) and by R01 DC014997 (J.T.L). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

All procedures listed immediately below in the Protocol section were conducted as described in protocols approved by the Washington University in St. Louis Institutional Animal Care and Use Committee.
1. Anesthetic Induction and Monitoring of Vital Signs
NOTE: This study used pigmented NIH-strain guinea pigs obtained from an in-house breeding colony.
<ol>
	<li>Use guinea pigs of either sex, weighing at least 350 g.</li>
	<li>Place the guinea pig in a neonatal warming isolette and give a ketamine/xylazine mixture intraperitoneally (50 mg/kg ketamine and 10 mg/kg xylazine) for induction anesthesia. Observe the guinea pig until it loses the toe-pinch reflex.</li>
	<li>Once loss of toe-pinch reflexes occurs, shave the posterior neck and head of the guinea pig with a hair trimmer typically advertised for human use.</li>
	<li>Inject a subcutaneous&#160;bolus of 12 mL of lactated Ringer&#39;s solution into the back of the animal.</li>
	<li>Place the guinea pig supine on a warming pad with legs raised and place 27.5 G butterfly needle intraperitoneally. Verify that the butterfly needle is in the correct position in the intraperitoneal space by ensuring only air is aspirated. If blood or fluid is aspirated, there is concern for delivery into the vascular or bowel system.&#160;The butterfly needle is used for repeated administration of anesthesia.</li>
	<li>Flip the guinea pig over to the prone position and secure the head to a stereotactic holder.</li>
	<li>Secure&#160;a pulse oximeter to the foot. If using pigmented guinea pigs, pigmented paws can prevent reading oxygen saturation. Therefore, place the pulse oximeter on any paw that is not pigmented.</li>
	<li>Insert a rectal temperature probe to monitor body temperature. The rectal probe is part of a warming blanket system that maintains body temperature at 38 &#176;C. Do not turn on the warming blanket until the rectal probe is in place to avoid overheating the warming blanket. If having difficulty placing the rectal probe, it can be laid alongside the guinea pig&#39;s body.</li>
	<li>Apply lubricant to both eyes of the guinea pig to prevent corneal abrasions.</li>
	<li>Administer supplemental oxygen as needed via a rubber tubing positioned near the nose to maintain oxygen saturation levels above 90%.</li>
	<li>Give enrofloxacin 0.5 mg/kg subcutaneously as an antibiotic prophylaxis.</li>
	<li>Give 0.25 mg/kg bupivacaine with 1:100,000 epinephrine subcutaneously at the anticipated incision site for local anesthesia and vasoconstrictive effects.</li>
	<li>Provide maintenance anesthesia every 20 min&#160;for 4 cycles and then only as needed based on vital signs. Routinely monitor the depth of anesthesia by body temperature, respiration rate, oxygen saturation, and heart rate.</li>
	<li>Monitor vital signs every 15 minutes (temperature, respiratory rate, heart rate, and oxygen saturation).</li>
</ol>
2. Surgical Preparation
<ol>
	<li>Once the head of the guinea pig is positioned securely in a stereotactic holder, place a piece of masking tape over the back to provide adequate tension along the skin overlying the occiput. Secure the ends of the tape to the stereotactic holder.</li>
	<li>Thoroughly prepare the skin overlying the occiput and posterior neck with povidone-iodine followed by 70% ethanol using aseptic technique, with three alternating antiseptic applications.</li>
	<li>At this point, use sterile precautions and autoclaved instruments. Place sterile drapes over the guinea pig.</li>
</ol>
3. Surgical Procedure
<ol>
	<li>Using a 15 blade, make a small, midline incision along the posterior occiput extending down into the posterior neck. Once under the skin, use iris scissors to detach the right posterior cervical muscles from the occipital bone. If any bleeding occurs while cutting the muscles, control by applying pressure with a sterile cotton ball.</li>
	<li>Using a combination of a #3 mm, #2 mm, and #1 mm diamond burr with a 5-0 suction and sterile irrigation, perform a craniotomy that is bounded by the external occipital crest, lamboidal ridge, the occipitomastoid suture line, and the dorsal margin of the foramen magnum.
	<ol>
		<li>Gently place a small piece of saline-moistened cotton ball&#160;under the bone while separating the occipital bone from the dura.</li>
	</ol>
	</li>
	<li>Skeletonize the sigmoid sinus with a #0.5 mm diamond burr and carefully remove the bone overlying it.</li>
	<li>Once the sigmoid sinus is exposed, gently retract the sigmoid sinus medially using a cotton ball and switch to using a 3-0 suction.</li>
	<li>Identify the operculum as a slit like structure that is located within the petrous temporal bone. The subarcuate fossa will be situated superiorly and the sigmoid sinus will be medial to it. The extra-osseous portion of the endolymphatic sac is then visualized as a clear sac entering the operculum and attached to the dura overlying the sigmoid sinus. The operculum is oval shaped, approximately 3 to 4 mm by 1.5 to 2 mm. However, as seen from the surgical view, the operculum appears as an approximate 1 mm slit. The visible portion of the sac from the surgical view is approximately the same size as the visible portion of the operculum, if not smaller.</li>
	<li>Apply gentle retraction to the sigmoid sinus medially in order to clearly visualize the extra-osseous portion of the endolymphatic sac and increase the tension between the extraosseous and intraosseous portions of the endolymphatic sac.
	<ol>
		<li>Use a fine angled pick to gently expunge the intermediate portion of the endolymphatic sac. It is critical that the expungement process leave no visible connection between the dura and the operculum; then place a fine pick inside the operculum to broadly scrape along the inside of the bone to injure it.</li>
		<li>Turn the fine pick in the direction of the endolymphatic duct and blindly disrupt the lining. At this point some bleeding may occur from a vessel within the operculum. It can be controlled with a small piece of cotton.</li>
	</ol>
	</li>
	<li>Dry the empty operculum with a small piece of cotton. Using the 3-0 suction as needed to keep the cotton dry.</li>
	<li>Obtain bone dust by using a small curette to scrape along the squamosal portion of the temporal bone. Generously pack the operculum with bone dust. Use a cotton ball and suction to keep the area dry while packing it with bone dust.</li>
	<li>Apply bone wax to the operculum to seal it. Ensure that there is no excess bone wax dislodged into the skull.</li>
	<li>Use bone wax to cover the skull&#160;defect.</li>
	<li>Approximate the posterior cervical muscles with 4-0 braided, absorbable suture in an interrupted fashion.</li>
	<li>Perform a subcuticular closure using a 4-0 braided, absorbable suture.</li>
</ol>
4. Post-procedure Care
<ol>
	<li>Remove the guinea pig from the custom stereotactic holder and transfer to a warming isolette.</li>
	<li>Give 2 mg/kg Atipamezole and 24 mL of lactated Ringer&#39;s solution (subcutaneously away from the incision). Give lactated Ringer&#39;s solution due to the diuretic effects of xylazine. Administer 0.2 mg/kg meloxicam subcutaneously for post-operative analgesic coverage.</li>
	<li>Obtain vital signs every 15 minutes until the guinea pig fully emerges from anesthesia.</li>
	<li>Give an additional 12 mL fluid bolus of lactated Ringer&#39;s solution about 2 hours from end of surgery during the recovery period.</li>
	<li>Once the guinea pig is alert, ambulating, voiding, and having bowel movements, return the guinea pig to the animal facility. Approximately 2 to 4 hours are needed for the guinea pig to emerge completely from anesthesia.</li>
	<li>Monitor guinea pigs twice daily for the first three post-operative days.&#160;If signs of discomfort are observed, administer 0.2 mg/kg meloxicam subcutaneously every 24 hours as needed.&#160;Alternatively, buprenorphine (0.05 mg/kg) may be administered subcutaneously if symptoms are not sufficiently managed by meloxicam.&#160;</li>
	<li>Give a 12 mL fluid bolus of lactated Ringer&#39;s solution subcutaneously twice a day for up to&#160;three days until the guinea pig reaches the pre-operative weight. If the guinea pig reaches its pre-operative weight prior to the third post-operative day, then stop fluid boluses. If the guinea pig continues to lose weight after the first three days, use a supplement nutrition shake typically advertised for human consumption mixed with crushed guinea pig food pellets.</li>
	<li>Monitor guinea pigs weekly until their end point.</li>
</ol>

<ol><li>Kimura, R. S., Schuknecht, H. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Membranous+Hydrops+in+the+Inner+Ear+of+the+Guinea+Pig+after+Obliteration+of+the+Endolymphatic+Sac%5BTitle%5D)+AND+%22Pract+oto-rhino-laryng%22%5BJournal%5D)">Membranous Hydrops in the Inner Ear of the Guinea Pig after Obliteration of the Endolymphatic Sac.</a> Pract oto-rhino-laryng. 27, 343-354 (1965).</li>
<li>Salt, A. N., DeMott, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Time+course+of+endolymph+volume+increase+in+experimental+hydrops+measured+in+vivo+with+an+ionic+volume+marker%5BTitle%5D)+AND+%22Hearing+Research%22%5BJournal%5D)">Time course of endolymph volume increase in experimental hydrops measured in vivo with an ionic volume marker.</a> Hearing Research. 74 (1-2), 165-172 (1994).</li>
<li>Walsted, A., Garbarsch, C., Michaels, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effect+of+craniotomy+and+cerebrospinal+fluid+loss+on+the+inner+ear.+An+experimental+study%5BTitle%5D)+AND+%22Acta+Oto-Laryngologica%22%5BJournal%5D)">Effect of craniotomy and cerebrospinal fluid loss on the inner ear. An experimental study.</a> Acta Oto-Laryngologica. 114 (6), 626-631 (1994).</li>
<li>Andrews, J. C., Bohmer, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+surgical+approach+to+the+endolymphatic+sac+and+the+cochlear+aqueduct+in+the+guinea+pig%5BTitle%5D)+AND+%22American+Journal+of+Otolaryngology%22%5BJournal%5D)">The surgical approach to the endolymphatic sac and the cochlear aqueduct in the guinea pig.</a> American Journal of Otolaryngology. 10 (1), 61-66 (1989).</li>
<li>Lee, J. R., Wright, C. G., Meyerhoff, W. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Modified+occipital+approach+to+the+endolymphatic+sac+and+cochlear+aqueduct+of+the+guinea+pig%5BTitle%5D)+AND+%22American+Journal+of+Otolaryngology%22%5BJournal%5D)">Modified occipital approach to the endolymphatic sac and cochlear aqueduct of the guinea pig.</a> American Journal of Otolaryngology. 14 (2), 165-169 (1993).</li>
<li>Megerian, C. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Surgical+induction+of+endolymphatic+hydrops+by+obliteration+of+the+endolymphatic+duct%5BTitle%5D)+AND+%22Journal+of+Visualized+Experiments%22%5BJournal%5D)">Surgical induction of endolymphatic hydrops by obliteration of the endolymphatic duct.</a> Journal of Visualized Experiments. (35), (2010).</li>
<li>Lichtenhan, J. T., Cooper, N. P., Guinan, J. J. Jr <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+new+auditory+threshold+estimation+technique+for+low+frequencies%3A+proof+of+concept%5BTitle%5D)+AND+%22Ear+and+Hearing%22%5BJournal%5D)">A new auditory threshold estimation technique for low frequencies: proof of concept.</a> Ear and Hearing. 34 (1), 42-51 (2013).</li>
<li>Lichtenhan, J. T., Hartsock, J., Dornhoffer, J. R., Donovan, K. M., Salt, A. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Drug+delivery+into+the+cochlear+apex%3A+Improved+control+to+sequentially+affect+finely+spaced+regions+along+the+entire+length+of+the+cochlear+spiral%5BTitle%5D)+AND+%22Journal+of+Neuroscience+Methods%22%5BJournal%5D)">Drug delivery into the cochlear apex: Improved control to sequentially affect finely spaced regions along the entire length of the cochlear spiral.</a> Journal of Neuroscience Methods. 273, 201-209 (2016).</li>
<li>Lichtenhan, J. T., Hartsock, J. J., Gill, R. M., Guinan, J. J. Jr, Salt, A. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+auditory+nerve+overlapped+waveform+%28ANOW%29+originates+in+the+cochlear+apex%5BTitle%5D)+AND+%22Journal+of+the+Association+for+Research+in+Otolaryngology%22%5BJournal%5D)">The auditory nerve overlapped waveform (ANOW) originates in the cochlear apex.</a> Journal of the Association for Research in Otolaryngology. 15 (3), 395-411 (2014).</li>
<li>Lichtenhan, J. T., Hirose, K., Buchman, C. A., Duncan, R. K., Salt, A. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Direct+administration+of+2-Hydroxypropyl-Beta-Cyclodextrin+into+guinea+pig+cochleae%3A+Effects+on+physiological+and+histological+measurements%5BTitle%5D)+AND+%22PloS+One%22%5BJournal%5D)">Direct administration of 2-Hydroxypropyl-Beta-Cyclodextrin into guinea pig cochleae: Effects on physiological and histological measurements.</a> PloS One. 12 (4), e0175236(2017).</li>
<li>Schneider, C. A., Rasband, W. S., Eliceiri, K. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((NIH+Image+to+ImageJ%3A+25+years+of+image+analysis%5BTitle%5D)+AND+%22Nature+Methods%22%5BJournal%5D)">NIH Image to ImageJ: 25 years of image analysis.</a> Nature Methods. 9 (7), 671-675 (2012).</li>
</ol>

The authors have nothing to disclose.

The presented extradural method had a success rate of 86% in achieving histologically confirmed endolymphatic hydrops and low-frequency hearing loss. The method reliably achieved histological evidence of endolymphatic hydrops by post-operative day 30, consistent with prior studies that used an intradural approach2. The significance of the method with respect to existing methods is that a CSF leak is not required, thus removing a potential confounding variable that has been suggested to result in a...

Endolymphatic hydrops is an enlargement of scala media. The presence of endolymphatic hydrops can be measured using the cross-sectional area of scala media. It is thought that clinical endolymphatic hydrops can be associated with low-frequency sensorineural hearing loss, such as that seen in Meniere&#39;s disease.&#160;But the origin(s) of the hearing loss remain unclear. To adequately study the origins of low-frequency hearing loss associated with endolymphatic hydrops, a reliable model is needed.
In 1965, Kimura and Schuknecht described how to induce endolymphatic hydrops in the guinea pig using an intradural approach1. Their technique involved using a posterior cranial fossa approach to access the operculum and subarcuate fossa. The steps involved incising dura, retracting the cerebellum with a Ringer&#39;s solution soaked cotton pad, and drilling across the endolymphatic duct and the intermediate portion of the endolymphatic sac. Bone wax was then placed into the operculum to separate the endolymphatic duct from the distal endolymphatic sac. The craniotomy defect was closed by placing absorbable gelatin powder (e.g., Gelfoam) and reapproximating the overlying muscles. Histologic evidence of endolymphatic hydrops was consistently found at post-operative days 1, 3, 7, 14, 21, and 30, demonstrating that the intradural approach was a reliable method&#160;to induce histologically-confirmed endolymphatic hydrops. Using the same intradural approach as Kimura and Schuknecht, but with different time points, Salt and DeMott confirmed that scala media in the second turn of the cochlea was significantly enlarged at day 4 and beyond2. While the actual morbidity of inducing a cerebrospinal fluid (CSF) leak using Kimura and Schuknecht&#39;s intradural approach was not reported in the original study, the presence of a CSF leak could increase the risk of meningitis. It has been suggested that loss of CSF could lead to an outflow of perilymph, resulting in a simultaneous temporary expansion of the endolymphatic volume in the guinea pig3. An extradural approach to inducing endolymphatic&#160;hydrops would be a safer option.
In 1989, Andrews and Bohmer described two extradural surgical approaches to reach the endolymphatic sac and duct, via either a middle cranial fossa approach or posterior cranial fossa approach, to obliterate the endolymphatic sac4. They described removing the operculum with a diamond drill, and then either drilling off the intermediate portion of the endolymphatic sac or using a fine pick to disrupt the endolymphatic sac and duct. In 1993, Lee, Wright, and Meyerhoff described a similar approach, which included drilling through the endolymphatic sac and duct, but differed in that they also simultaneously obstructed the cochlear aqueduct5. They demonstrated the presence of endolymphatic hydrops, as assessed via histology, at four weeks after obliterating the endolymphatic sac and obstructing the cochlear aqueduct. Megerian et al. was the first to publish a video article demonstrating an extradural obliteration of the endolymphatic sac and duct that involved drilling directly on the medial portion of the operculum to enter into the endolymphatic sac and duct6. They demonstrated histologic evidence of endolymphatic hydrops in a guinea pig sacrificed at 28 weeks after surgery, as well as hearing loss in the 16 kHz region6. Whether it was possible to induce histologically confirmed endolymphatic hydrops and low-frequency hearing loss at an early time point using extradural approaches was unknown.
The overall goal of this report is to demonstrate an extradural approach to induce experimental endolymphatic hydrops at 30 days post-operatively by obliterating the endolymphatic sac and injuring the endolymphatic duct with a fine pick. The rationale behind the use of this technique is the advantage of avoiding the need to drill on the petrous temporal bone, thereby removing the risk of accidentally injuring the dura and causing a CSF leak, mitigating the possibility of injuring the posterior semicircular canal, and reducing the risk of injury to the sigmoid sinus.

<table><tbody><tr><td>12 mL syringe</td><td>Henke-Sass Wolf</td><td>5100-X00V0</td><td></td></tr><tr><td>1 mL and 3 mL syringe</td><td>BD Precision(Ordered from Fischer Sci)</td><td>14-826-87 15859152</td><td></td></tr><tr><td>27.5 butterfly gauge needle</td><td>Terumo Surflo Winged Infusion Set, Terumo Corporation, Japan) (Ordered from McKesson)</td><td>448407</td><td></td></tr><tr><td>4-0 suture</td><td>McKesson</td><td>1034507</td><td></td></tr><tr><td>4 x 4 gauze sponges</td><td>Dukal (Ordered form McKesson)</td><td>374454</td><td></td></tr><tr><td>60 mL syringe</td><td>Fisher Sci</td><td>22-031-375</td><td></td></tr><tr><td>Anspach otologic drill</td><td>Anspach</td><td>SC2100</td><td></td></tr><tr><td>atipamezole</td><td>Zoetis</td><td>107204-6</td><td></td></tr><tr><td>autoclave</td><td>Fisher sci</td><td>&lt;a href=&quot;https://www.fishersci.com/shop/products/fisher-scientific-isotemp-general-purpose-heating-drying-ovens/151030508&quot;&gt;15-103-0508&lt;/a&gt;</td><td></td></tr><tr><td>autoclave bags</td><td>McKesson</td><td>524881</td><td></td></tr><tr><td>bayonet separator</td><td>Olympus</td><td>AL 130564</td><td></td></tr><tr><td>bupivicaine</td><td>auro Medics Pharma</td><td>555150-169-10</td><td></td></tr><tr><td>clear sterile drape</td><td>3M</td><td>1020</td><td></td></tr><tr><td>cotton balls</td><td>Fisherbrand (ordered from Fisher Sci)</td><td>22-456-885</td><td></td></tr><tr><td>cotton swabs</td><td>McKesson</td><td>508716</td><td></td></tr><tr><td>diamond burrs #3, #2, #1, and #0.5 mm</td><td>Anspach</td><td>QD8-3SD; QD8-2SD; QD8-1SD; QD8-05SD</td><td></td></tr><tr><td>diaper pad</td><td>McKesson</td><td>945330</td><td></td></tr><tr><td>disposable 15 blade</td><td>Swann-Morton</td><td>0305</td><td></td></tr><tr><td>enrofloxacin</td><td>Hospira</td><td>0409-4888-01</td><td></td></tr><tr><td>epinephrine</td><td>McKesson</td><td>&lt;a href=&quot;https://www.findacode.com/code.php?set=NDC&amp;amp;c=63739-0456&quot;&gt;63739-0456&lt;/a&gt;</td><td></td></tr><tr><td>eye ointment</td><td>Dechra Vet Products</td><td>17033-211-38</td><td></td></tr><tr><td>Freer elevator</td><td>Grace Medical</td><td>215100FX</td><td></td></tr><tr><td>gelfoam</td><td>Pfizer (Ordered from McKesson)</td><td>82830</td><td></td></tr><tr><td>hair trimmers</td><td>Oster Power Pro Cordless (ordered from Amazon)</td><td>078400-020-000</td><td></td></tr><tr><td>iodine scrub</td><td>Purdue Pharma (ordered from mcKesson)</td><td>521243</td><td></td></tr><tr><td>iris scissors</td><td>Olympus</td><td>CL-542114</td><td></td></tr><tr><td>ketamine</td><td>Henry Schein Animal Health</td><td>55853</td><td></td></tr><tr><td>lactated ringers</td><td>B. Braun Medical (ordered from McKesson)</td><td>186662</td><td></td></tr><tr><td>lancet knife by Rosen</td><td>Grace Medical</td><td>151100FX</td><td>referred to as curette in the text</td></tr><tr><td>lubricant</td><td>Milex (ordered from Cooper Surgical)</td><td>MX5030</td><td></td></tr><tr><td>masking tape</td><td>3M (ordered from fisher sci</td><td>19047259</td><td></td></tr><tr><td>metal rectangle basin</td><td>Amazon</td><td>B07NQDBC6T</td><td></td></tr><tr><td>needle holder</td><td>Olympus</td><td>CR 213015-ENT</td><td></td></tr><tr><td>needles: 27 gage, 18 gauge</td><td>BD Precision(Ordered from Fischer Sci)</td><td>&lt;a href=&quot;https://www.fishersci.com/shop/products/bd-general-use-precisionglide-hypodermic-needles-20/1482648&quot;&gt;14-826-48 14-826-5D&lt;/a&gt;</td><td></td></tr><tr><td>neonatal warming isollete</td><td>Air Borne Life Support Systems</td><td>731-1800</td><td></td></tr><tr><td>operating microscope</td><td>Carl Zeiss</td><td>OPMI pico</td><td></td></tr><tr><td>oxygen tank</td><td>AirGas</td><td>OX USP200</td><td></td></tr><tr><td>pulse ox</td><td>CapnoTrue (Ordered from Medacx)</td><td>M-3090112001</td><td></td></tr><tr><td>rectal probe with heating blanket</td><td>Harvard Apparatus</td><td>probe: PY2 50-7217 Heating Blanket: PY2 50-7214</td><td></td></tr><tr><td>red body holder</td><td>Lichtenhan Lab</td><td>N/A</td><td>In-house product</td></tr><tr><td>right angle</td><td>Olympus</td><td>BV-230337</td><td></td></tr><tr><td>rosen needle</td><td>Olympus</td><td>AM-130566</td><td>customized, it is the instrument I use to tear the sac</td></tr><tr><td>rubber tubing for O&lt;sub&gt;2&lt;/sub&gt; administration</td><td>Fisher Sci</td><td>14-171-104</td><td></td></tr><tr><td>saran wrap</td><td>Fisher Sci</td><td>NC9617977</td><td></td></tr><tr><td>stereotactic head holder</td><td>WUSTL Instrument Machine Shop</td><td>N/A</td><td>In-house product</td></tr><tr><td>sterile drapes</td><td>Cardinal Health</td><td>7553</td><td></td></tr><tr><td>suction tube by Baron</td><td>Grace Medical</td><td>034903FX 034905FX</td><td>#3 and #5 Suction</td></tr><tr><td>tissue forceps adson brown</td><td>Grace Medical</td><td>325112FX</td><td></td></tr><tr><td>Weitlander retractor</td><td>Olympus Grace Medical</td><td>BL200011 100313FX</td><td></td></tr><tr><td>xylazine</td><td>Akorn</td><td>59399-110-20</td><td></td></tr></tbody></table>

a revised surgical approach to induce endolymphatic hydrops in the guinea pig

A Revised Surgical Approach to Induce Endolymphatic Hydrops in the Guinea Pig

Endolymphatic hydrops is an enlargement of scala media that is most often associated with Meniere&#39;s disease, though the pathophysiologic mechanism(s) remain unclear. In order to adequately study the attributes of endolymphatic hydrops, such as the origins of low-frequency hearing loss, a reliable model is needed. The guinea pig is a&#160;good model because it hears in the low-frequency regions that are putatively affected by endolymphatic hydrops. Previous research has demonstrated that endolymphatic hydrops can be induced surgically via intradural or extradural approaches that involve drilling on the endolymphatic duct and sac. However, whether it was possible to create an endolymphatic hydrops model using an extradural approach that avoided dangerous drilling on the endolymphatic duct and sac was unknown. The objective of this study was to demonstrate a revised extradural approach to induce experimental endolymphatic hydrops at 30 days post-operatively by obliterating the endolymphatic sac and injuring the endolymphatic duct with a fine pick. The sample size consisted of seven guinea pigs. Functional measurements of hearing were made and temporal bones were subsequently harvested for histologic analysis. The approach had a success rate of 86% in achieving endolymphatic hydrops. The risk of cerebrospinal fluid leak was minimal. No perioperative deaths or injuries to the posterior semicircular canal occurred in the sample. The presented method demonstrates a safe and reliable way to induce endolymphatic hydrops at a relatively quick time point of 30 days. The clinical implications are that the presented method provides a reliable model to further explore the origins of low-frequency hearing loss that can be associated endolymphatic hydrops.

The presented method used an extradural approach to obliterate the endolymphatic sac and injure the endolymphatic duct with a fine pick in seven guinea pigs consisting of two males and five females. The average duration of surgery was 2 hours from incision to closure. The total drill time ranged from 5-10 minutes. Up to 4 hours was needed for the guinea pig to fully emerge from anesthesia. There were no intra-operative or post-operative deaths in the sample. There were no injuries to the ...

This article demonstrates an extradural approach to obliterate the guinea pig endolymphatic sac and injure the endolymphatic duct with a fine pick in order to induce experimental endolymphatic hydrops.

Endolymphatic hydrops is an enlargement of scala media that is most often associated with Meniere&#39;s disease, though the pathophysiologic mechanism(s) ...

Using this procedure, endolymphatic hydrops can be induced relatively quickly in guinea pigs with an adequate success rate. The main advantage of this technique is that endolymphatic hydrops can be induced with minimal risk for CSF leak or injury to the posterior semicircular canal. After confirming a lack of response to pedal reflex, inject a 12 milliliter subcutaneous fluid bolus of lactated Ringer's solution into the back of the anesthetized guinea pig and place the guinea pig in the supine position on a warming pad.Place a 27.5 gauge butterfly needle intraperitoneally confirming a correct placement by the presence of air only within the needle tip and place the guinea pig in the prone position. Secure the head in a stereotactic holder and secure a pulse oximeter onto an unpigmented foot. Use a rectal probe and a warming blanket to maintain the body temperature at 38 degrees Celsius and apply ointment to the animal's eyes.Place a piece of masking tape over the back to provide adequate tension along the skin overlying the occiput and fix the ends of the tape to the stereotactic holder. Then liberally prep the skin overlying the occiput and posterior neck with three consecutive iodine scrubs and 70%alcohol and place a sterile drape over the animal. Begin the surgical procedure by using a number 15 blade to make a small midline incision along the posterior occiput extending down to the posterior neck.Once under the skin, use iris scissors to detach the right posterior cervical muscles from the occipital bone, controlling any bleeding with pressure from a sterile cotton ball. Using a dissecting microscope and a combination of a number three, two, and one millimeter diamond burrs with a 5-0 suction and sterile irrigation, perform a craniectomy that is bounded as far as the external occipital crest laterally, lambdoidal ridge superiorly, the occipitomastoid suture line medially, and the dorsal margin of the foramen magnum inferiorly. And use a 0.5 millimeter diamond burr to skeletonize the sigmoid sinus.Gently place a small piece of saline moistened cotton under the bone while separating the occipital bone from the dura. Carefully remove the bone overlying the sinus and use a cotton ball to gently retract the sigmoid sinus medially. Carefully retract the sigmoid sinus and identify the operculum as a slit-like structure located within the petrous temporal bone.The extraosseous portion of the endolymphatic sac can be visualized as a clear sac-like connection between the operculum and the dura overlying the sigmoid sinus. Apply gentle medial retraction to the sigmoid sinus to clearly visualize the extraosseous portion of the endolymphatic sac and increase the tension between the extraosseous and intraosseous portions of the sac. Using a fine angled pick, gently expunge the intermediate portion of the endolymphatic sac and broadly scrape a fine pick inside the operculum along the inside of the bone.It's critical to the success of this procedure that no visible connection between the dura and the operculum remain. Use a small curette to scrape along the temporal bone to obtain bone chips and generously pack the operculum with the bone chips. Seal the operculum with bone wax taking care that there is no excess wax dislodged into the skull and use bone wax to cover the craniectomy defect.Then approximate the posterior cervical muscles with 4-0 braided absorbable sutures in an interrupted fashion and perform a two-layer closure by approximating the deep layer with simple interrupted sutures and the epidermal layer using a subcuticular closure with a 4-0 braided absorbable suture. In this representative experiment, histologic analysis of the temporal bones revealed endolymphatic hydrops throughout the right cochlea compared to the left cochlea in six out of seven guinea pigs. Quantification of the cross-sectional area of the scala media across each turn indicated that the cross-sectional area was generally larger in the ears of animals that survived 30 days after obliteration of the endolymphatic sac compared to that observed in control animals.Auditory nerve overlapped waveform thresholds were increased in animals with endolymphatic hydrops demonstrating the presence of low-frequency hearing loss while most high-frequency cochlear compound action potential thresholds between eight and 20 kilohertz were within the normal range. If the area between the dura and the operculum is dry after expunging the sac, try placing additional retraction on the sigmoid sinus to make sure there's no visible connection between it and the operculum. Following this procedure, additional studies on cochlear anatomy and physiology can be performed to address important research questions about endolymphatic hydrops.

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Research

JoVE Journal

Neuroscience

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