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Journal
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Biology
/
果蝇
幼虫的欧诺细胞的解剖和脂滴染色
JoVE Journal
Biology
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JoVE Journal
Biology
Dissection and Lipid Droplet Staining of Oenocytes in
Drosophila
Larvae
Please note that all translations are automatically generated.
Click here for the English version.
果蝇
幼虫的欧诺细胞的解剖和脂滴染色
DOI:
10.3791/60606-v
•
05:37 min
•
December 28, 2019
•
Chuanxian Wei*
1
,
Yan Yan*
1
,
Xiaoli Miao
,
Renjie Jiao
2,3
1
Sino-French Hoffmann Institute, School of Basic Medical Sciences
,
Guangzhou Medical University
,
2
The Second Affiliated Hospital of Guangzhou Medical University
,
3
The State Key Laboratory of Respiratory Disease
,
Guangzhou Medical University
Chapters
00:04
Title
00:32
Egg Laying
01:13
Starvation Treatment
02:10
Oenocyte Dissection
03:10
Lipid Droplet Staining
04:02
Mounting and Imaging
04:41
Results: Representative Lipid Droplet Staining
05:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里介绍了使用BODIPY 493/503(一种脂滴特异性荧光染料)对
果蝇
幼虫中的卵虫细胞进行解剖和脂滴染色的详细方法。
Tags
Oenocyte
Lipid Droplet
Drosophila
Larvae
Dissection
Staining
Starvation
Metabolism
Epidermis
PBS
Fixation Buffer
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