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Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
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Biochemistry
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JoVE Journal Biochemistry
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

DOI:
10.3791/60616-v

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08:56 min

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March 08, 2020

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, , ,
1Department of Biology,Concordia University, 2Department of Chemistry and Biochemistry, Centre for Biological Applications of Mass Spectrometry,Concordia University

Chapters

  • 00:05Introduction
  • 00:34Yeast Culture and Lipid Extraction
  • 03:55Extracted Lipid Separation
  • 05:26Mass Spectrometric Analysis and Lipid Class Identification and Quantification
  • 06:53Results: Representative Quantitative Analysis of Saccharomyces Cerevisiae Cellular Lipidomes
  • 08:19Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol using liquid chromatography coupled with tandem mass spectrometry to identify and quantify major cellular lipids in Saccharomyces cerevisiae. The described method for a quantitative assessment of major lipid classes within a yeast cell is versatile, robust, and sensitive.

Tags

Keywords: LipidomeSaccharomyces CerevisiaeLiquid ChromatographyTandem Mass SpectrometryLipid ExtractionYeast CultureInternal Lipid StandardsQuantitative Analysis

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