Screening for <em>Thermotoga maritima</em> Membrane-Bound Pyrophosphatase Inhibitors

Keni Vidilaseris; Niklas G. Johansson; Ainoleena Turku; Alexandros Kiriazis; Gustav Boije af Genn&#228;s; Jari Yli-Kauhaluoma; Henri Xhaard; Adrian Goldman

doi:10.3791/60619

Journal

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Biochemistry

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Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

Screening for <em>Thermotoga maritima</em> Membrane-Bound Pyrophosphatase Inhibitors

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

6,612 Views

•

09:11 min

•

November 23, 2019

DOI:

10.3791/60619-v

DOI:

10.3791/60619-v

•

09:11 min

November 23, 2019

•

6606 Views

Keni Vidilaseris*¹, Niklas G. Johansson*², Ainoleena Turku², Alexandros Kiriazis², Gustav Boije af Gennäs², Jari Yli-Kauhaluoma², Henri Xhaard², Adrian Goldman^1,3

¹Research Program in Molecular and Integrative Biosciences,University of Helsinki ²Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy,University of Helsinki ³School of Biomedical Sciences and Astbury Centre for Structural Molecular Biology,University of Leeds

Summary

Here we present a screening method for membrane-bound pyrophosphatase (from Thermotoga maritima) inhibitors based on the molybdenum blue reaction in a 96 well plate format.

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Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

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Summary

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