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JoVE Journal
Bioengineering

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Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy
 

Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy

Article doi: 10.3791/60784
January 25th, 2020 Usage Statistics

Summary January 25th, 2020

We present a protocol for immobilizing single macromolecules in microfluidic devices and quantifying changes in their conformations under shear flow. This protocol is useful for characterizing the biomechanical and functional properties of biomolecules such as proteins and DNA in a flow environment.

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