5,812 Views
•
08:47 min
•
January 25, 2020
DOI:
Summary
Automatically generated
We present a protocol for immobilizing single macromolecules in microfluidic devices and quantifying changes in their conformations under shear flow. This protocol is useful for characterizing the biomechanical and functional properties of biomolecules such as proteins and DNA in a flow environment.
Related Videos
00:10
Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
Related Videos
8192 Views
15:10
From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
Related Videos
11412 Views
11:26
Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
Related Videos
9298 Views
20:00
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
Related Videos
13905 Views
07:56
Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time
Related Videos
3153 Views
08:02
Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles
Related Videos
2598 Views
10:59
Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET
Related Videos
3169 Views
11:55
Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
Related Videos
15042 Views
10:19
Single-Molecule FRET Imaging for Observing the Conformational Dynamics of Dynamin-Like GTPase Atlastin
Related Videos
415 Views
10:23
Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
Related Videos
222 Views
Read Article
Cite this Article
Pisapati, A. V., Wang, Y., Blauch, M. E., Wittenberg, N. J., Cheng, X., Zhang, X. F. Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy. J. Vis. Exp. (155), e60784, doi:10.3791/60784 (2020).
Copy