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Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy
Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy

Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy

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08:47 min

January 25, 2020

DOI:

08:47 min
January 25, 2020

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Summary

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We present a protocol for immobilizing single macromolecules in microfluidic devices and quantifying changes in their conformations under shear flow. This protocol is useful for characterizing the biomechanical and functional properties of biomolecules such as proteins and DNA in a flow environment.

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