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Development of a Larval Zebrafish Infection Model for Clostridioides difficile
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Developmental Biology
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JoVE Journal Developmental Biology
Development of a Larval Zebrafish Infection Model for Clostridioides difficile

Development of a Larval Zebrafish Infection Model for Clostridioides difficile

DOI:
10.3791/60793-v

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09:13 min

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February 14, 2020

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, , , ,
1Division of Cellular and Molecular Neurobiology, Zoological Institute,Technische Universität Braunschweig, 2Department of Molecular Biotechnology,Turkish-German University, 3Institut für Mikrobiologie,Technische Universität Braunschweig, 4Braunschweig Integrated Centre of Systems Biology, 5Helmholtz Centre for Infection Research

Chapters

  • 00:05Introduction
  • 00:36Preparation and Labeling of C. difficile and Spores with Fluorescent Dye
  • 01:47Injection of Stained C. difficile into Zebrafish Larvae
  • 03:40Gavage of Zebrafish Larvae and Confocal Microscopy Analysis
  • 05:28Dissection of Larval Zebrafish Intestine to Recover Viable C. difficile
  • 06:35Results: Infection of C. difficile in Zebrafish
  • 08:27Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a safe and effective method to infect zebrafish larvae with fluorescently labeled anaerobic C. difficile by microinjection and noninvasive microgavage.

Tags

Keywords: Larval ZebrafishClostridioides DifficileInfection ModelInnate Immune SystemMacrophagesAnaerobic BacteriaOral AdministrationOD600Fluorescent DyeLow-melting AgaroseDanieau’s MediumTricainePhenol RedMicroinjectionGastrointestinal Tract

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