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Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy
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JoVE Journal Chemistry
Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy

Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy

DOI:
10.3791/60849-v

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06:00 min

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May 27, 2022

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, , , , , ,
1University Grenoble Alpes,INSERM UA7, Synchrotron Radiation for Biomedicine (STROBE), 2CNRS, UMR 5254, Université Pau & Pays Adour, E2S UPPA,Institut des Sciences Analytiques et de Physicochimie pour l’Environnement et les Matériaux, 3Institut Jacques Monod, UMR 7592 CNRS,Université Paris Diderot, 4OSUG, UMS 832 CNRS,Université Grenoble Alpes, 5FAME and FAME-UHD beamlines,ESRF, the European Synchrotron, 6ID16A beamline,ESRF, the European Synchrotron

Chapters

  • 00:05Introduction
  • 00:51Human PC-3 and OVCAR-3 Cancer Cell Pellet Preparation
  • 02:58High Resolution X-Ray Absorption Spectroscopy (HERFD-XAS) Measuring Procedure
  • 03:40Results: Selenium (Se) and Iron (Fe) K-Edge Typical HERFD-XANES Analyses
  • 04:56Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents a detailed procedure to prepare biological cryosamples for synchrotron-based X-ray absorption spectroscopy experiments. We describe all the steps required to optimize sample preparation and cryopreservation with examples of the protocol with cancer and phytoplankton cells. This method provides a universal standard of sample cryo-preparation.

Tags

Keywords: Biological SamplesSpeciationCryogenic TemperatureX-ray Absorption SpectroscopyCell PelletsSelenium TreatmentCell CultureCancer Cell LinesSample PreparationCentrifugationCryosampleSynchrotron

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