We describe protocols for assessing the degree of transduction by cell-penetrating peptides utilizing ex vivo imaging systems followed by paraffin embedding, sectioning, and confocal fluorescent microscopy using cardiac targeting peptide as an example. In our protocol, a single animal can be used to acquire both types of imaging assessment of the same organs, thereby cutting the number of animals needed for studies by half.
Feldman, K. S., Zahid, M. In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide. J. Vis. Exp. (160), e60895, doi:10.3791/60895 (2020).