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JoVE Journal
Biology

This content is Open Access.

In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide
 

In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide

Article doi: 10.3791/60895
June 11th, 2020

Summary June 11th, 2020

We describe protocols for assessing the degree of transduction by cell-penetrating peptides utilizing ex vivo imaging systems followed by paraffin embedding, sectioning, and confocal fluorescent microscopy using cardiac targeting peptide as an example. In our protocol, a single animal can be used to acquire both types of imaging assessment of the same organs, thereby cutting the number of animals needed for studies by half.

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