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In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide
In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide

In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide

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09:02 min

June 11, 2020

DOI:

09:02 min
June 11, 2020

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Summary

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We describe protocols for assessing the degree of transduction by cell-penetrating peptides utilizing ex vivo imaging systems followed by paraffin embedding, sectioning, and confocal fluorescent microscopy using cardiac targeting peptide as an example. In our protocol, a single animal can be used to acquire both types of imaging assessment of the same organs, thereby cutting the number of animals needed for studies by half.

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