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June 11, 2020
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We describe protocols for assessing the degree of transduction by cell-penetrating peptides utilizing ex vivo imaging systems followed by paraffin embedding, sectioning, and confocal fluorescent microscopy using cardiac targeting peptide as an example. In our protocol, a single animal can be used to acquire both types of imaging assessment of the same organs, thereby cutting the number of animals needed for studies by half.
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Cite this Article
Feldman, K. S., Zahid, M. In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide. J. Vis. Exp. (160), e60895, doi:10.3791/60895 (2020).
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