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I Vivo Billeddannelse af transduktionseffektivitet ved hjertemålretning af peptid
JoVE Journal
Biology
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JoVE Journal Biology
In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide

I Vivo Billeddannelse af transduktionseffektivitet ved hjertemålretning af peptid

DOI:
10.3791/60895-v

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09:02 min

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June 11, 2020

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1Department of Developmental Biology,University of Pittsburgh School of Medicine

Chapters

  • 00:04Introduction
  • 00:41Cardiac Targeting Peptide (CTP) Delivery and Organ Harvest
  • 02:10In Vivo Imaging
  • 04:17Histology
  • 07:20Results: Representative In Vivo CTP Transduction Efficiency Imaging
  • 08:24Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver protokoller til vurdering af graden af transduktion ved celle-gennemtrængende peptider udnytte ex vivo billeddannelse systemer efterfulgt af paraffin indlejring,sektion, og konfokal fluorescerende mikroskopi ved hjælp af hjerte-målretning peptid som et eksempel. I vores protokol kan et enkelt dyr bruges til at erhverve begge typer billeddiagnostisk vurdering af de samme organer og derved halvere antallet af dyr, der er nødvendige til undersøgelser.

Tags

In Vivo ImagingTransduction EfficiencyCardiac Targeting PeptideBio-distributionCy5.5CD1 MouseEx Vivo Optical ImagingFluorescence ImagingRadiant EfficiencyBackground Subtraction

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