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JoVE Journal Neuroscience
Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion
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Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Article DOI: 10.3791/60939-v • 09:09 min • April 12th, 2020
April 12th, 2020 •
1,2,4, 1,2,4, 1,3, 1,2,4, 3, 1,2,4, 1,2,4
1Dept. of Neurosurgery, University of Michigan Medical School, 2Dept. of Cell and Developmental Biology, University of Michigan Medical School, 3Dept. of Ophthalmology & Visual Science, University of Michigan Medical School, 4Rogel Cancer Center, University of Michigan Medical School

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Summary
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Laser microdissection (LMD) is a sensitive and highly reproducible technique that can be used to uncover pathways that mediate glioma heterogeneity and invasion. Here, we describe an optimized protocol to isolate discrete areas from glioma tissue using laser LMD followed by transcriptomic analysis.

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Laser Capture Microdissection Glioma Subregions Spatial Characterization Molecular Characterization Intratumoral Heterogeneity Oncostreams Invasion RNA Sequencing MRNA Expression Mesenchymal Cells Tumor Invasion Tissue Histology RNA Integrity Laser Microdissection Samples Tumor Morphology Heterogeneity Brain Tumor Tissue Anatomical Areas Molecular Profiles Mechanistic Knowledge Tumor Progression Therapeutic Targets Bioluminescence Mouse Tumor Burden Oxygenated Tyrode Solution Sucrose Solution
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