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JoVE Journal Bioengineering
Una plataforma de microscopía multifoto personalizada para imágenes en vivo de cornea de ratón y conjuntiva
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Una plataforma de microscopía multifoto personalizada para imágenes en vivo de cornea de ratón y conjuntiva

Article DOI: 10.3791/60944-v • 06:53 min • May 17th, 2020
May 17th, 2020 •
1, 1, 2,3, *1,3,4,5, *6,7
1Department of Biomedical Engineering, National Taiwan University, 2Institute of Pharmacology, College of Medicine, National Taiwan University, 3Molecular Imaging Center, National Taiwan University, 4Department of Dermatology, National Taiwan University Hospital, and College of Medicine, 5Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, 6Department of Ophthalmology, Chang Gung Memorial Hospital, 7College of Medicine, Chang Gung University
* These authors contributed equally

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Aquí se presenta una plataforma microscópica multifoto para imágenes de superficie ocular de ratón en vivo. El ratón fluorescente transgénico permite la visualización de núcleos celulares, membranas celulares, fibras nerviosas y capilares dentro de la superficie ocular. Las señales no lineales de segunda generación armónica derivadas de estructuras de collagenounous proporcionan imágenes sin etiquetas para arquitecturas estromales.

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Bioingeniería Número 159 córnea conjuntiva superficie ocular microscopía multifotónica MPM imágenes intravitales ratón transgénico
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