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Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice
JoVE Journal
Behavior
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JoVE Journal Behavior
Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice

DOI:
10.3791/61023-v

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14:40 min

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October 27, 2020

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, ,
1Biology and Chemistry, Synthetic Biology,University of Bremen, 2Advanced Fluorescence Microscopy, Faculty of Biology and Biotechnology,Ruhr-Universität Bochum

Chapters

  • 00:00Introduction
  • 00:59Preparation of the Optical Implant
  • 01:51Injection and Implantation
  • 06:01Setting up a New Experiment in the Video Tracking Software
  • 09:07Open Field Experiment (Anxiety)
  • 09:57Barnes Maze (Learning)
  • 10:47Data Analysis (example Open Field)
  • 12:21Data Analysis (example Open Field): Statistics Program
  • 13:23Representative Results
  • 13:52Conclusion

Summary

Automatic Translation

Automatic Translation

With optogenetic manipulation of specific neuronal populations or brain regions, behavior can be modified with high temporal and spatial resolution in freely moving animals. By using different optogenetic tools in combination with chronically implanted optical fibers, a variety of neuronal modulations and behavioral testing can be performed.

Tags

Keywords: OptogeneticsNeuronal ActivityBehaviorFreely Moving MiceOptical ImplantNeuronal ModulationCell Type-specificNeuronal CircuitryStereotactic SurgeryAnesthesiaImplantation

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