Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice

Franziska Bender; Tatiana Korotkova; Alexey Ponomarenko

doi:10.3791/57349

Journal

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Neuroscience

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Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice

11,687 Views

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07:33 min

•

June 29, 2018

DOI:

10.3791/57349-v

DOI:

10.3791/57349-v

•

07:33 min

June 29, 2018

•

11664 Views

Franziska Bender^1,2, Tatiana Korotkova^2,3, Alexey Ponomarenko^1,2

¹Systems Neurophysiology Research Group, Institute of Clinical Neuroscience and Medical Psychology, Medical Faculty,Heinrich Heine University Düsseldorf ²Behavioural Neurodynamics Group,Leibniz Institute for Molecular Pharmacology (FMP)/ NeuroCure Cluster of Excellence ³Neuronal Circuits and Behavior Research Group,Max Planck Institute for Metabolism Research

Summary

We describe the use of optogenetics and electrophysiological recordings for selective manipulations of hippocampal theta oscillations (5-10 Hz) in behaving mice. The efficacy of the rhythm entrainment is monitored using local field potential. A combination of opto- and pharmacogenetic inhibition addresses the efferent readout of hippocampal synchronization.