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1Systems Neurophysiology Research Group, Institute of Clinical Neuroscience and Medical Psychology, Medical Faculty,Heinrich Heine University Düsseldorf, 2Behavioural Neurodynamics Group,Leibniz Institute for Molecular Pharmacology (FMP)/ NeuroCure Cluster of Excellence, 3Neuronal Circuits and Behavior Research Group,Max Planck Institute for Metabolism Research
Chapters
- 00:04Title
- 00:39Viral Injection
- 01:52Preparation of Optic Fibers
- 02:45Preparation of Tungsten Wire Arrays for LFP Recordings
- 03:56Stereotaxic Implantation
- 05:26Optogenetic Stimulation and Electrophysiological Data Acquisition
- 06:15Results: Optogenetic Entrainment of Theta Rhythm
- 07:02Conclusion
We describe the use of optogenetics and electrophysiological recordings for selective manipulations of hippocampal theta oscillations (5-10 Hz) in behaving mice. The efficacy of the rhythm entrainment is monitored using local field potential. A combination of opto- and pharmacogenetic inhibition addresses the efferent readout of hippocampal synchronization.
