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Biology

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A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT
 

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

Article DOI: 10.3791/61081-v 07:10 min June 12th, 2020
June 12th, 2020

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Summary

We employed a geological (coring) sampling protocol to procure cortical bone specimens of uniform size for SRµCT experiments from the anterior aspect of human femora. This method is minimally destructive, efficient, results in cylindrical specimens that minimize imaging artifacts from irregular sample shapes and improves microarchitectural visualization and analysis.

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Sectioning Coring Image Processing High-throughput Cortical Bone Sample Procurement Synchrotron Micro-CT Bone Specimen Methodology Dimensions Cylindrical Shape Data Sets Quality Cost-effective Rocks Fossils Hard Material Uniformly Sized Cores High-pressure Experiments Rheology Conical Shapes Bone Dust Drill Bit Coring Speed Instructional Texts Videos Bone Preparation Tutorial Glass Microscope Slide Thermal Epoxy Resin
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