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Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature
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JoVE Journal Biology
Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

DOI:
10.3791/50162-v

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11:49 min

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April 05, 2013

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, , , ,
1U.S. Department of Agriculture, 2Department of Viticulture and Enology,University of California – Davis, 3Hawkesbury Institute for the Environment,University of Western Sydney, 4Advanced Light Source,Lawrence Berkeley National Lab, 5Citrus Research & Education Center,University of Florida

Chapters

  • 00:05Title
  • 01:47Sample Preparation for Live Plants
  • 04:24Sample Preparation for Fresh, Excised Plant Tissue
  • 05:44Sample Preparation for Dried Woody Tissues
  • 06:58Scanning the Sample in the 8.3.2 Hutch
  • 08:44Data Processing, Visualization, and Quantification
  • 10:29Results: Plant Structure Images from the HRCT
  • 11:23Conclusion

Summary

Automatic Translation

Automatic Translation

High resolution x-ray computed tomography (HRCT) is a non-destructive diagnostic imaging technique that can be used to study the structure and function of plant vasculature in 3D. We demonstrate how HRCT facilitates exploration of xylem networks across a wide range of plant tissues and species.

Tags

Keywords: High Resolution Computed TomographyHRCT3D VisualizationPlant VasculatureXylemSynchrotron X-rayPlant AnatomyWoody PlantsHerbaceous PlantsNon-destructive Imaging

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