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Simpel påvisning af primær cilier ved immunofluorescens
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Simpel påvisning af primær cilier ved immunofluorescens

Article DOI: 10.3791/61155-v • 08:07 min • May 15th, 2020
May 15th, 2020 •
1, 2, 3, 4, 1, 1
1Department of Radiobiology, Faculty of Military Health Sciences in Hradec Kralove, University of Defence, 2Department of Clinical Biochemistry and Diagnostics, University Hospital, 3Department of Oncology, Thomayer Hospital, Charles University, 4Department of Oncology and Radiotherapy, Faculty of Medicine, Charles University

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Primære cilier er ekstracellulære strukturer forbundet med centriole. Primær cilia detektion ved immunfluorescerende farvning er en forholdsvis enkel procedure, der resulterer i ekstremt høj kvalitet billeder. I denne protokol, fibroblaster udtrykke primære cilier blev fastsat, immunstained, og afbildet i en fluorescerende eller konfokal mikroskop.

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Biologi primær cilier immunofluorescens fibroblaster acetylerede alfa tubulin gamma tubulin mikroskopi dyrkning in vitro serum sult bestråling doxorubicin taxol.
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