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Enkel Detektion av primär Cilier genom immunofluorescens
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Enkel Detektion av primär Cilier genom immunofluorescens

Article DOI: 10.3791/61155-v • 08:07 min • May 15th, 2020
May 15th, 2020 •
1, 2, 3, 4, 1, 1
1Department of Radiobiology, Faculty of Military Health Sciences in Hradec Kralove, University of Defence, 2Department of Clinical Biochemistry and Diagnostics, University Hospital, 3Department of Oncology, Thomayer Hospital, Charles University, 4Department of Oncology and Radiotherapy, Faculty of Medicine, Charles University

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Primära cilier är extracellulära strukturer som är associerade med centriole. Primära cilier upptäckt av immunofluorescent färgning är en relativt enkel procedur som resulterar i extremt högkvalitativa bilder. I detta protokoll, fibroblaster uttrycker primära cilier var fast, immunostained och avbildas i en fluorescerande eller confocal mikroskop.

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Biologi primära cilier immunofluorescens fibroblaster acetylerad alfa tubulin gamma tubulin mikroskopi odling in vitro serum svält bestrålning doxorubicin taxol.
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