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Journal / Biology / Vibratome-Cut Miyokardiyal Dilimlerden İnsan…
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JoVE Journal Biology
Vibratome-Cut Miyokardiyal Dilimlerden İnsan Ventriküler Kardiyomiyositlerin İzolasyon
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Vibratome-Cut Miyokardiyal Dilimlerden İnsan Ventriküler Kardiyomiyositlerin İzolasyon

Article DOI: 10.3791/61167-v • 11:19 min • May 10th, 2020
May 10th, 2020 •
1, 1,2, 1,2
1Institute of Cellular and Molecular Physiology, Friedrich-Alexander University Erlangen-Nürnberg, 2Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg

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Sunulan vibratome kesilmiş miyokardiyal dilimlerden insan ve hayvan ventriküler kardiyomiyosit izolasyon için bir protokoldür. Kalsiyuma dayanıklı hücrelerin yüksek verimi (200 hücre/mg'a kadar) az miktarda dokudan (<50 mg) elde edilebilir. Protokol soğuk iskemiye maruz kalan miyokardiyum için 36 saate kadar geçerlidir.

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Biyoloji Sayı 159 insan kalp yetmezliği miyokardiyal dilimler kardiyomiyosit izolasyonu vibratom kontraktil miyositler kalsiyum görüntüleme kardiyak uyarma-kontraksiyon kaplin
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