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Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve
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Neuroscience
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JoVE Journal Neuroscience
Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve

Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve

DOI:
10.3791/61264-v

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09:52 min

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August 31, 2020

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, , ,
1Department of Neuroscience,The Ohio State University, 2Present address: Interdepartmental Neuroscience Graduate Program and Department of Neurobiology,Northwestern University, 3Quantitative Biology Institute,Ohio University, 4Department of Physics and Astronomy,Ohio University

Chapters

  • 00:04Introduction
  • 00:41Initial Assembly of Nerve Perfusion Chamber
  • 01:26Dissection and Preparation of Mouse Tibial Nerve
  • 03:09Final Nerve Perfusion Chamber Assembly
  • 04:48Fluorescence Activation and Image Acquisition
  • 06:21Flatfield and Darkfield Image Acquisition
  • 07:37Results: Pulse-escape and Pulse-spread Experiments
  • 09:17Conclusion

Summary

Automatic Translation

Automatic Translation

We describe fluorescence photoactivation methods to analyze the axonal transport of neurofilaments in single myelinated axons of peripheral nerves from transgenic mice that express a photoactivatable neurofilament protein.

Tags

Keywords: Neurofilament TransportFluorescence MicroscopyPhotoactivatable Fluorescent FusionMyelinated AxonsTibial NerveNerve DissectionPerfusion ChamberOxygenated SalineMicrodissectionNerve Imaging

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