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Imaging and Analysis of Oil Red O-Stained Whole Aorta Lesions in an Aneurysm Hyperlipidemia Mouse Model

Article DOI: 10.3791/61277-v • 07:57 min • May 2nd, 2022
May 2nd, 2022 •
1, 2, 1,3
1Yale Cardiovascular Research Center, Department of Internal Medicine, Yale University School of Medicine, 2Department of Surgery, Yale University School of Medicine, 3Department of Cell Biology, Yale University School of Medicine

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This protocol provides a step-by-step procedure to analyze atherosclerotic burden in mice. Investigators can use this protocol to compare the abundance, location, and size of atherosclerotic lesions in different animals.

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Imaging Analysis Oil Red O-Stained Whole Aorta Lesions Aneurysm Hyperlipidemia Mouse Model Quantification Atherosclerotic Lesions ApoE Mice Hyperlipidemic Mice Genetic Factors New Therapies Protocol Step-by-step Instructions Atherosclerotic Burden Qualitative Analysis Quantitative Analysis Single Mouse Inducing Atherosclerosis DPBS Syringe Needle Abdominal Skin Fine Scissors Ribcage Thoracic Cavity Right Atrium Of The Heart Apical Left Ventricular Puncture Liver And Kidney Color Change Saline Perfusion Nonwoven Sponge Cleaning Chest Cavity Wiping Non-experimental Organs Removal
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